The efficacy of chlorine-based disinfectants against planktonic and biofilm bacteria for decentralised point-of-use drinking water
The efficacy of chlorine-based disinfectants against planktonic and biofilm bacteria for decentralised point-of-use drinking water
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DOI：
10.1038/s41545-021-00139-w
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发表时间：
2021-11-04
期刊：
影响因子：
11.4
通讯作者：
Reynolds, Darren M.
中科院分区：
文献类型：
--
作者：
Clayton, Gillian E.;Thorn, Robin M. S.;Reynolds, Darren M.
氯溶液广泛用于生产生物安全饮用水。在集中处理系统和分配网络不实用的地方，使用点（POU）饮用水处理系统的能力引起了人们的兴趣。本研究考察了三种氯基消毒剂（次氯酸离子[OCl-]、次氯酸[HOCl]和电化学活化溶液[ECAS]）在POU饮用水中的抗菌和抗生物膜活性。以大肠杆菌为检测对象，比较了两种菌的相对抑菌活性。在疾病控制中心[CDC]生物膜反应器中，利用已建立的固定式铜绿假单胞菌比较了抗生物膜活性。在有机负载（牛血清蛋白）存在的情况下，HOCl对浮游大肠杆菌的抑菌活性最高，浓度为50 mg L-1。然而，在>= 50 mg L-1游离氯时，ECAS对铜绿假单胞菌生物膜的抗生物膜活性显著高于OCl-和HOCl。基于这一证据，HOCl是主要氯种的消毒剂（HOCl和ECAS）将是POU饮用水应用中合适的氯基消毒剂替代品。
Chlorine solutions are used extensively for the production of biologically safe drinking water. The capability of point-of-use [POU] drinking water treatment systems has gained interest in locations where centralised treatment systems and distribution networks are not practical. This study investigated the antimicrobial and anti-biofilm activity of three chlorine-based disinfectants (hypochlorite ions [OCl-], hypochlorous acid [HOCl] and electrochemically activated solutions [ECAS]) for use in POU drinking water applications. The relative antimicrobial activity was compared within bactericidal suspension assays (BS EN 1040 and BS EN 1276) using Escherichia coli. The anti-biofilm activity was compared utilising established sessile Pseudomonas aeruginosa within a Centre for Disease Control [CDC] biofilm reactor. HOCl exhibited the greatest antimicrobial activity against planktonic E. coli at >50 mg L-1 free chlorine, in the presence of organic loading (bovine serum albumen). However, ECAS exhibited significantly greater anti-biofilm activity compared to OCl- and HOCl against P. aeruginosa biofilms at >= 50 mg L-1 free chlorine. Based on this evidence disinfectants where HOCl is the dominant chlorine species (HOCl and ECAS) would be appropriate alternative chlorine-based disinfectants for POU drinking water applications.