Photoresponsive DNA Nanocapsule Having an Open/Close System for Capture and Release of Nanomaterials
Photoresponsive DNA Nanocapsule Having an Open/Close System for Capture and Release of Nanomaterials
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DOI：
10.1002/chem.201404757
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发表时间：
2014-11-10
影响因子：
4.3
通讯作者：
Sugiyama, Hiroshi
中科院分区：
文献类型：
--
作者：
Takenaka, Tomohiro;Endo, Masayuki;Sugiyama, Hiroshi
设计并制备了一种光功能化的方形双锥DNA纳米胶囊（NC），用于纳米材料载体的制备。在NC中引入了光可控的开/关系统和支点系统，通过光照射和链置换来包合和释放金纳米粒子（AuNP）。通过凝胶电泳和原子力显微镜（AFM）检测了可逆的开闭状态，并通过高速AFM直接观察了开闭行为。通过特异性DNA链（捕获链）的杂交进行NC内DNA修饰的AuNP的包封，并且通过添加含有支点的互补DNA链（释放链）来检查AuNP的释放。通过打开NC和随后的链置换实现AuNP从NC的释放。
A photofunctionalized square bipyramidal DNA nanocapsule (NC) was designed and prepared for the creation of a nanomaterial carrier. Photocontrollable open/close system and toehold system were introduced into the NC for the inclusion and release of a gold nanoparticle (AuNP) by photoirradiation and strand displacement. The reversible open and closed states were examined by gel electrophoresis and atomic force microscopy (AFM), and the open behavior was directly observed by high-speed AFM. The encapsulation of the DNA-modified AuNP within the NC was carried out by hybridization of a specific DNA strand (capture strand), and the release of the AuNP was examined by addition of toehold-containing complementary DNA strand (release strand). The release of the AuNP from the NC was achieved by the opening of the NC and subsequent strand displacement.