Circulating levels of hormones, lipids, and immune mediators in post-traumatic stress disorder - a 3-month follow-up study
Circulating levels of hormones, lipids, and immune mediators in post-traumatic stress disorder - a 3-month follow-up study
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DOI：
10.3389/fpsyt.2015.00049
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发表时间：
2015-04-14
影响因子：
4.7
通讯作者：
Vidovic, Andelko
中科院分区：
文献类型：
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作者：
Jergovic, Mladen;Bendelja, Kreso;Vidovic, Andelko
许多外周血分析物已被提议作为创伤后应激障碍（PTSD）的潜在生物标志物。很少有研究调查观察到的生物标志物的变化是否会随着时间的推移而持续存在。本研究的目的是调查与战斗相关的慢性 PTSD 与一系列假定的 PTSD 生物标志物的关联，并确定测量的可靠性，即随时间的相关性。患有慢性创伤后应激障碍 (PTSD) 的克罗地亚退伍军人 (n= 69) 和年龄匹配的健康对照组 (n= 32) 均为男性，在相隔 3 个月的两个时间点进行评估。测定了脂质、皮质醇、硫酸脱氢表雄酮 (DHEA-S)、催乳素和 C 反应蛋白的血清水平。使用多重测定同时评估血清中的 13 种分析物：细胞因子 [干扰素-γ、白介素 (IL)-1 β、IL-2、IL-4、IL-6、TNF-α]、粘附分子（sPECAM-1、sICAM-1）、趋化因子（IL-8 和 MIP-1 α）、sCD40L、神经生长因子和瘦素。通过参数或非参数检验（包括协方差的重复测量分析）来检验组间差异和随时间的变化。还计算了可靠性估计 [组内相关系数 (ICC) 和 kappa]。发现 PTSD 与较高水平的 DHEA-S F(1,75) = 8.14, p= 0.006)] 和较低水平的催乳素 F(1,75) = 5.40, p = 0.023] 存在密切关联。测量结果显示出良好至极好的重现性（DHEA-S，ICC = 0.50；催乳素，ICC = 0.79）。各组之间的血清脂质没有差异，但在 PTSD 组中观察到 3 个月后 LDLC 显着增加（t = 6.87，p < 0.001）。 1158 在 PTSD 组中较低（t = 4.37，p < 0.001），但评估显示重复性较差（ICC = -0.08）。稳定的 DHEA-S 和催乳素变化凸显了它们作为 PTSD 可靠标记的潜力。 3个月后血脂谱的变化表明PTSD患者可能更容易出现高脂血症。某些变量的高个体差异强调了纵向研究在 PTSD 生物标志物研究中的重要性。
A number of peripheral blood analytes have been proposed as potential biomarkers of post-traumatic stress disorder (PTSD). Few studies have investigated whether observed changes in biomarkers persist over time. The aim of this study was to investigate the association of combat-related chronic PTSD with a wide array of putative PTSD biomarkers and to determine reliability of the measurements, i.e., correlations over time. Croatian combat veterans with chronic PTSD (n= 69) and age-matched healthy controls (n= 32), all men, were assessed at two time points separated by 3 months. Serum levels of lipids, cortisol, dehydroepiandrosterone-sulfate (DHEA-S), prolactin, and C-reactive protein were determined. Multiplex assay was used for the simultaneous assessment of 13 analytes in sera: cytokines [interferon-gamma, interleukin (IL)-1 beta, IL-2, IL-4, IL-6, TNF-alpha], adhesion molecules (sPECAM-1, sICAM-1), chemokines (IL-8 and MIP-1 alpha), sCD40L, nerve growth factor, and leptin. Group differences and changes over time were tested by parametric or nonparametric tests, including repeated measures analysis of covariance. Reliability estimates [intraclass correlation coefficient (ICC) and kappa] were also calculated. Robust associations of PTSD with higher levels of DHEA-S F(1,75) = 8.14, p= 0.006)] and lower levels of prolactin F(1,75) = 5.40, p = 0.023] were found. Measurements showed good to excellent reproducibility (DHEA-S, ICC = 0.50; prolactin, ICC = 0.79). Serum lipids did not differ between groups but significant increase of LDLC after 3 months was observed in the PTSD group (t = 6.87, p < 0.001). 1158 was lower in the PTSD group (t = 4.37, p < 0.001) but assessments showed poor reproducibility (ICC = -0.08). Stable DHEA-S and prolactin changes highlight their potential to be reliable markers of PTSD. Change in lipid profiles after 3 months suggests that PTSD patients may be more prone to hyperlipidemia. High intraindividual variability in some variables emphasizes the importance of longitudinal studies in investigations of PTSD biomarkers.