Temperature-sensitive yeast mutants defective in mitochondrial inheritance.
Temperature-sensitive yeast mutants defective in mitochondrial inheritance.
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DOI：
10.1083/jcb.111.3.967
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发表时间：
1990-09
影响因子：
7.8
通讯作者：
Yaffe, M P
中科院分区：
文献类型：
--
作者：
McConnell, S J;Stewart, L C;Talin, A;Yaffe, M P
线粒体向子细胞的分布是有丝分裂细胞生长的基本特征，但促进这种线粒体遗传的分子机制尚不清楚。我们已经分离出酿酒酵母的突变体，它们对线粒体转移到生长的芽中是温度敏感的。这些突变体中有两个含有单一的隐性核突变，mdm1和mdm2，导致温度敏感性的增长和异常的线粒体分布在非允许的温度。间接免疫荧光显微镜和透射电子显微镜证实了突变体细胞芽中线粒体的缺失。mdm1损伤也延缓核分裂并阻止核转移到芽中。含有mdm2突变的细胞在不允许的温度下生长，依次形成多个芽，每个芽都有一个细胞核，但没有线粒体。mdm1或mdm2都不影响液泡物质转移到芽中，也不引起微管蛋白或肌动蛋白基细胞骨架的明显变化。mdm1和mdm2突变是细胞周期特异性的，在G1晚期或S早期显示执行点。
The distribution of mitochondria to daughter cells is an essential feature of mitotic cell growth, yet the molecular mechanisms facilitating this mitochondrial inheritance are unknown. We have isolated mutants of Saccharomyces cerevisiae that are temperature- sensitive for the transfer of mitochondria into a growing bud. Two of these mutants contain single, recessive, nuclear mutations, mdm1 and mdm2, that cause temperature-sensitive growth and aberrant mitochondrial distribution at the nonpermissive temperature. The absence of mitochondria from the buds of mutant cells was confirmed by indirect immunofluorescence microscopy and by transmission electron microscopy. The mdm1 lesion also retards nuclear division and prevents the transfer of nuclei into the buds. Cells containing the mdm2 mutation grown at the nonpermissive temperature sequentially form multiple buds, each receiving a nucleus but no mitochondria. Neither mdm1 or mdm2 affects the transfer of vacuolar material into the buds or causes apparent changes in the tubulin- or actin-based cytoskeletons. The mdm1 and mdm2 mutations are cell-cycle specific, displaying an execution point in late G1 or early S phase.