An autoradiographic study of alpha 1-adrenergic receptors in the brain of the Japanese quail (Coturnix coturnix japonica).
An autoradiographic study of alpha 1-adrenergic receptors in the brain of the Japanese quail (Coturnix coturnix japonica).
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日本鹌鹑 (Coturnix coturnix japonica) 大脑中 α1-肾上腺素能受体的放射自显影研究。
DOI：
10.1007/bf00218868
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发表时间：
1989
影响因子：
3.6
通讯作者：
McEwen,BS
中科院分区：
文献类型：
--
作者：
Balthazart,J;Ball,GF;McEwen,BS
以特异性拮抗剂[3H]吡唑嗪为配体，采用体外定量放射自显像技术研究了日本鹌鹑α1-肾上腺素能受体的神经解剖分布。通过饱和分析发现存在可饱和（Bmax<200 fmol/mg蛋白）高亲和力（Kd< 0.12 nM）的结合位点。在纹状体/腹侧部、海马、梨状皮质、背外侧皮质区、丘脑背侧和前掌核中检测到高浓度的[3H]吡唑嗪结合位点。在丘间核、外侧隔、下丘脑后丘和结节处可见较低浓度。在视前区和下丘脑前区几乎没有看到结合。在端脑结构中发现的相对较多的结合位点与先前的哺乳动物研究一致。这与哺乳动物下丘脑前部的模式相反，据报道，下丘脑前部的一些核含有高受体密度。
The neuroanatomical distribution ofα1-adrenergic receptors was studied in Japanese quail by quantitative in vitro autoradiography using the specific antagonist [3H]prazosin as the ligand. The presence of saturable (Bmax<200 fmol/mg protein) high affinity (Kd< 0.12 nM) binding sites was detected by saturation analysis. High concentrations of [3H]prazosin binding sites were detected in the archistriatum/pars ventralis, the hippocampus, the cortex piriformis, the area corticoidea dorsolateralis, the dorsal thalamus, and the nucleus praetectalis. Lower concentrations were seen in the intercollicular nucleus, the lateral septum, and the posterior and tuberal hypothalamus. Very little binding was seen in the preoptic and anterior hypothalamic areas. The relatively high number of binding sites identified in the telencephalic structures agrees well with previous mammalian studies. This is in contrast with the pattern in the anterior hypothalamus where, in mammals, a number of nuclei have been reported to contain a high receptor density.