Physicochemical characteristics and anticoagulant activities of low molecular weight fractions by free-radical depolymerization of a fucosylated chondroitin sulphate from sea cucumber Thelenata ananas
Physicochemical characteristics and anticoagulant activities of low molecular weight fractions by free-radical depolymerization of a fucosylated chondroitin sulphate from sea cucumber Thelenata ananas
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DOI：
10.1016/j.foodchem.2010.03.042
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发表时间：
2010-10-01
期刊：
影响因子：
8.8
通讯作者：
Ding, Hui
中科院分区：
文献类型：
--
作者：
Wu, Mingyi;Xu, Shimin;Ding, Hui
开发了一种通过自由基解聚来自Thelenata ananas的新型岩藻糖基化硫酸软骨素的工艺。得到了具有不同分子量和窄分子量分布的级分。通过理化性质、红外光谱和核磁共振谱对产物进行了表征。结果证实了解聚后级分的一级结构的保留。以活化部分凝血活酶时间（APTT）为指标，评价各组分的抗凝血活性。APTT活性随着类胰蛋白酶功能而与分子量成比例地降低，并且增强APTT活性的延长需要至少约6-8个单位（6000-7000 Da）的寡糖链。与高剂量的低分子量肝素（LMWH）相比，更理想的分子量部分（13，950 Da）显示出显著较低的抗凝活性。因此，相对于LMWH，该馏分具有更大的潜力作为具有降低的出血风险的抗血栓形成剂。(C)2010爱思唯尔有限公司版权所有。
A process of depolymerization of a new fucosylated chondroitin sulphate from Thelenata ananas by free-radicals was developed. The fractions with different molecular weights and a narrow molecular weight distribution was obtained. The products were characterised by physicochemical properties, FT-IR spectra and NMR spectra. The results confirm the retention of primary structure of the fractions after the depolymerization. Furthermore, the anticoagulant activity of the fractions was evaluated by the activated partial thromboplastin time (APTT). The APTT activity decreases in proportion to the molecular weight following a logarithmic-like function, and the potentiating of prolongation of APTT activity requires at least an oligosaccharide chain of about 6-8 units (6000-7000 Da). Compared to high doses of low molecular weight heparin (LMWH), a more desirable molecular weight fraction (13,950 Da) demonstrates significantly lower anticoagulant activity. Thus, the fraction has more potential as an antithrombotic agent with reduced bleeding risk relative to LMWH. (C) 2010 Elsevier Ltd. All rights reserved.