Biofilm Growth of Chlorella Sorokiniana in a Rotating Biological Contactor Based Photobioreactor
Biofilm Growth of Chlorella Sorokiniana in a Rotating Biological Contactor Based Photobioreactor
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DOI：
10.1002/bit.25301
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发表时间：
2014-12-01
影响因子：
3.8
通讯作者：
Wijffels, R. H.
中科院分区：
文献类型：
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作者：
Blanken, W.;Janssen, M.;Wijffels, R. H.
微藻生物膜可作为微藻生物量的生产平台。本研究采用基于旋转生物接触器(RBC)的光生物反应器作为生物膜培养微藻生物量的生产平台。在被称为阿尔加迪斯克的光生物反应器中，微藻在部分淹没在生长介质中的垂直旋转圆盘上的生物膜中生长。目的是评估阿尔加迪克光生物反应器在圆盘粗糙度、圆盘转速和二氧化碳浓度方面的潜力。在实验室规模的阿尔加迪斯克系统中，这些目标被评估与生产力、光合作用效率和长期栽培稳定性的关系。虽然使用的是实验室规模的阿尔加迪克系统，但评估的操作参数与放大有关。以小球藻Sorokiniana为模型微藻。在实验室规模的阿尔加迪斯克反应器中，每天的生产率为20.1+/-0.7g/m(2)圆盘表面，光下的生物量产率为0.9+/-0.04g干重/摩尔光子。不同的圆盘转速对生物膜的生长和底物向生物膜内扩散的影响很小。然而，二氧化碳的限制大大降低了生产率，使生产率降至每天每米(2)磁盘表面2-4克。在没有重新接种阿尔加迪克的情况下，生产力可以保持在21周以上。在极端条件下，如PH值为9-10，温度高于40摄氏度，二氧化碳浓度较低，生产率会下降。然而，当恢复最佳栽培条件时，最大生产力迅速恢复。这些结果表明，如果扩散不限制二氧化碳供应，显然有机会大规模使用阿尔加迪克光生物反应器生产微藻生物量。(C)2014年威利期刊公司。
Microalgae biofilms could be used as a production platform for microalgae biomass. In this study, a photobioreactor design based on a rotating biological contactor (RBC) was used as a production platform for microalgae biomass cultivated in biofilm. In the photobioreactor, referred to as Algadisk, microalgae grow in biofilm on vertical rotating disks partially submerged in a growth medium. The objective is to evaluate the potential of the Algadisk photobioreactor with respect to the effects of disk roughness, disk rotation speed and CO2 concentration. These objectives where evaluated in relationship to productivity, photosynthetic efficiency, and long-term cultivation stability in a lab-scale Algadisk system. Although the lab-scale Algadisk system is used, operation parameters evaluated are relevant for scale-up. Chlorella Sorokiniana was used as model microalgae. In the lab-scale Algadisk reactor, productivity of 20.1 +/- 0.7 g per m(2) disk surface per day and a biomass yield on light of 0.9 +/- 0.04 g dry weight biomass per mol photons were obtained. Different disk rotation speeds did demonstrate minimal effects on biofilm growth and on the diffusion of substrate into the biofilm. CO2 limitation, however, drastically reduced productivity to 2-4 g per m(2) disk surface per day. Productivity could be maintained over a period of 21 weeks without re-inoculation of the Algadisk. Productivity decreased under extreme conditions such as pH 9-10, temperature above 40 degrees C, and with low CO2 concentrations. Maximal productivity, however, was promptly recovered when optimal cultivation conditions were reinstated. These results exhibit an apparent opportunity to employ the Algadisk photobioreactor at large scale for microalgae biomass production if diffusion does not limit the CO2 supply. (C) 2014 Wiley Periodicals, Inc.