Peucedanum japonicum extract attenuates allergic airway inflammation by inhibiting Th2 cell activation and production of pro-inflammatory mediators
Peucedanum japonicum extract attenuates allergic airway inflammation by inhibiting Th2 cell activation and production of pro-inflammatory mediators
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DOI：
10.1016/j.jep.2017.09.006
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发表时间：
2018-01-30
影响因子：
5.4
通讯作者：
Kwon, Bo-In
中科院分区：
文献类型：
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作者：
Chun, Jin Mi;Lee, A. Reum;Kwon, Bo-In
民族药理学相关性：在韩国和日本，Peucedanum japonicum Thunberg 的根传统上用于治疗咳嗽、感冒、头痛和炎症性疾病。其对过敏性肺部炎症的影响尚未得到研究。研究目的：使用哮喘小鼠模型和脂多糖 (LPS) 刺激的巨噬细胞系来研究前胡提取物 (PJE) 的抗哮喘作用。材料和方法：小鼠接受两轮卵清蛋白致敏，间隔 1 周，然后在第 13-16 天进行四次鼻内卵清蛋白激发。对照组仅接受生理盐水。两个卵清蛋白致敏组从第一次卵清蛋白致敏前 1 周开始每周 5 天口服给药媒介物或 PJE (200 mg/kg)。第三组在第 12-16 天口服哮喘药物孟鲁司特 (10 mg/kg)。第 17 天处死所有动物。评估肺部的组织学特征、炎症细胞浸润、Th2 细胞活化和 GATA 结合蛋白 3 (GATA-3) 表达。评估支气管肺泡灌洗液 (BALF) 的 2 型细胞因子水平。评估PJE对体外幼稚CD4(+)脾细胞Th2极化以及LPS刺激的RAW 264.7细胞产生促炎介质和细胞因子的影响。结果：PJE治疗抑制OVA诱导的肺部炎症细胞浸润、嗜酸性粒细胞增多、Th2激活和GATA-3表达，降低BALF中白细胞介素(IL)-5和IL-13水平，体外下调 Th2 激活，并抑制巨噬细胞诱导型一氧化氮、环氧合酶-2、肿瘤坏死因子-α 和 IL-6 的产生。结论：PJE 通过抑制 Th2 细胞激活和巨噬细胞炎症介质的产生来减轻过敏性气道炎症。前胡可能是过敏性肺部炎症的候选疗法。
Ethnopharmacolokical relevance: The root of Peucedanum japonicum Thunberg is traditionally used to treat coughs, colds, headache and inflammatory diseases in Korea and Japan. Its effects on allergic lung inflammation have not been investigated.Aim of the study: To investigate the anti-asthmatic effects of Peucedanum japonicum extract (PJE) using a murine model of asthma and a lipopolysaccharide (LPS)-stimulated macrophage cell line.Materials and methods: Mice underwent two rounds of sensitization with ovalbumin 1 week apart followed by four intranasal ovalbumin challenges on days 13-16. The control group received saline only. Two ovalbumin-sensitized groups were orally administered vehicle or PJE (200 mg/kg) 5 days a week starting 1 week before the first ovalbumin sensitization. The third group was orally administered the asthma medication Montelukast (10 mg/kg) on days 12-16. All animals were sacrificed on day 17. The lungs were assessed for histological features, inflammatory cell infiltration, Th2 cell activation and GATA-binding protein-3 (GATA-3) expression. The bronchoalveolar lavage fluid (BALF) was assessed for type 2 cytokine levels. The effect of PJE on the in vitro Th2 polarization of naive CD4(+) splenocytes and the production of pro-inflammatory mediators and cytokines by LPS-stimulated RAW 264.7 cells was evaluated.Results: PJE treatment inhibited OVA-induced inflammatory cell infiltration, eosinophilia, Th2 activation, and GATA-3 expression in the lung, reduced the interleukin (IL)-5 and IL-13 levels in BALF, down-regulated Th2 activation in vitro, and inhibited the macrophage production of inducible nitric oxide, cyclooxygenase-2, tumor necrosis factor-alpha, and IL-6.Conclusion: PJE attenuated allergic airway inflammation by inhibiting Th2 cell activation and macrophage production of inflammatory mediators. Peucedanum japonicum may be candidate therapy for allergic lung inflammation.