A STUDY OF BIOLOGICAL BEHAVIOR BASED ON THE EXPRESSION OF A PROLIFERATING ANTIGEN IN NEUROENDOCRINE TUMORS OF THE DIGESTIVE-SYSTEM
A STUDY OF BIOLOGICAL BEHAVIOR BASED ON THE EXPRESSION OF A PROLIFERATING ANTIGEN IN NEUROENDOCRINE TUMORS OF THE DIGESTIVE-SYSTEM
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DOI：
10.1159/000217749
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发表时间：
1992-01-01
期刊：
影响因子：
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通讯作者：
WILANDER, E
中科院分区：
文献类型：
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作者：
CHAUDHRY, A;OBERG, K;WILANDER, E
增殖抗原Ki-67免疫组化的表达进行了评估，在32个胃肠道类癌和5个胰岛细胞肿瘤。在组织切片中，计算每个肿瘤区域的标记细胞核数量。将肿瘤分类为低增殖（< 0.3标记细胞/mm 2）、中等增殖（0.3-1标记细胞/mm 2）和高增殖（> 1标记细胞/mm 2）。在26个肿瘤患者接受抗肿瘤治疗（α-干扰素）的增殖活性降低。在治疗的中肠类癌中，转移组织中的增殖活性显著降低（p < 0.05）。虽然没有统计学意义，但从未经治疗的患者中收集的原发性中肠类癌显示出比肝转移癌更低的增殖活性。生存分析显示，具有低增殖活性的肿瘤的患者比具有高增殖活性的患者具有更好的生存率（p < 0.05）。单细胞荧光DNA分析显示，在大多数未经治疗的患者（9/11例）的肿瘤中，有规则的二倍体干细胞系。计算的增殖活性和DNA图谱之间没有发现相关性。所获得的结果表明，增殖抗原的Ki-67免疫组化的表达，可用于评估消化系统的神经内分泌肿瘤的生物学行为和预测生存。
The expression of a proliferating antigen by Ki-67 immunohistochemistry was evaluated in 32 gastrointestinal carcinoids and in 5 pancreatic islet cell tumors. In the tissue sections the number of labelled nuclei was calculated per tumor area. The tumors were classified as low proliferating (< 0.3 labelled cells/mm2), medium proliferating (0.3-1 labelled cells/mm2), and high proliferating (> 1 labelled cell/mm2). In 26 tumors obtained from patients receiving antitumor therapy (alpha-interferon) the proliferative activity was decreased. In treated midgut carcinoids the proliferative activity in metastatic tissue was significantly reduced (p < 0.05). Though not statistically significant, primary midgut carcinoids collected from untreated patients displayed a lower proliferative activity than liver metastases. A survival analysis revealed that patients with tumors displaying low proliferative activity had a better survival than those with high proliferative activity (p < 0.05). Single cell cytofluorometric DNA analyses showed regular diploid stem cell lines in the majority of tumors from untreated patients (9/11 cases). No correlation was found between the calculated proliferative activity and the DNA profile. The obtained results indicate that the expression of a proliferation antigen by Ki-67 immunohistochemistry can be used to evaluate the biological behavior of neuroendocrine tumors of the digestive system and predict survival.