Bioorthogonal chemical tagging of protein cholesterylation in living cells
Bioorthogonal chemical tagging of protein cholesterylation in living cells
复制标题
DOI：
10.1039/c0cc04710d
复制
发表时间：
2011-01-01
影响因子：
4.9
通讯作者：
Tate, Edward W.
中科院分区：
文献类型：
--
作者：
Heal, William P.;Jovanovic, Biljana;Tate, Edward W.
我们报道了在活细胞中用叠氮化物标记翻译后胆固醇化蛋白的第一个生物正交化学探针。这使得蛋白质胆固醇化的快速多路荧光检测和亲和标记成为可能，例如Sonic hedgehog蛋白，为重新鉴定胆固醇化蛋白开辟了新的方法。
We report the first chemical probe for bioorthogonal chemical tagging of post-translationally cholesterylated proteins with an azide in living cells. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins.