A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse
A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse
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生物技术工艺可有效地从甘蔗渣中联合生产两种高附加值产品：葡萄糖和低聚木糖
DOI：
10.1016/j.biortech.2015.12.082
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发表时间：
2016-03-01
影响因子：
11.4
通讯作者：
Feng, Jia-Xun
中科院分区：
文献类型：
--
作者：
Xue, Jian-Long;Zhao, Shuai;Feng, Jia-Xun
在这项研究中，两个高附加值的产品，葡萄糖和低聚木糖（XOS）的联产，利用甘蔗渣（SB）的多产品生物炼油框架内的Box-Behnken设计为基础的响应面优化方法进行了研究。所开发的工艺导致去除木聚糖的SB的最大纤维素转化率为98.69 +/-1.30%，并且最大提取的SB木聚糖转化为XOS 57.36 +/-0.79%（木二糖和木三糖），这是文献中报道的最高SB木聚糖转化率，使用来自青霉菌EU 2106的纤维素酶和重组内切-β-1，巴斯德毕赤酵母中的4-木聚糖酶因此，质量平衡分析表明，葡萄糖和XOS的最大产量分别为34.43 +/- 0.32 g和5.96 +/- 0.09 g/100 g原料SB。总体而言，所述工艺可能是SB综合利用的首选方案。（C）2016 Elsevier Ltd.保留所有权利。
In this study, a co-production of two high value-added products, glucose and xylooligosaccharides (XOS), was investigated by utilizing sugarcane bagasse (SB) within a multi-product bio-refinery framework optimized by Box-Behnken design-based response surface methodology. The developed process resulted in a maximum cellulose conversion of xylan-removed SB, 98.69 +/- 1.30%, and a maximum extracted SB xylan conversion into XOS (xylobiose and xylotriose) of 57.36 +/- 0.79% that was the highest SB xylan conversion reported in the literature, employing cellulase from Penicillium oxalicum EU2106 and recombinant endo-beta-1,4-xylanase in Pichia pastoris. Consequently, a mass balance analysis showed that the maximum yields of glucose and XOS were 34.43 +/- 0.32 g and 5.96 +/- 0.09 g per 100 g raw SB. Overall, this described process may be a preferred option for the comprehensive utilization of SB. (C) 2016 Elsevier Ltd. All rights reserved.