Autoactivation of Translation Causes the Bloom of Prorocentrum donghaiense in Harmful Algal Blooms
Autoactivation of Translation Causes the Bloom of Prorocentrum donghaiense in Harmful Algal Blooms
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DOI：
10.1021/acs.jproteome.1c00051
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发表时间：
2021-05-06
影响因子：
4.4
通讯作者：
Zhang, Gong
中科院分区：
文献类型：
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作者：
Cao, Xin;Guo, Zhong;Zhang, Gong
有害藻华是生态系统失衡的表现，是世界范围内重大的海洋自然灾害之一，严重威胁着人类的健康。一些赤潮藻类的特殊基因组大小阻碍了对分子机制的基因组研究，如原心藻。本研究对东海原心菌进行翻译组测序（RNC-seq），在适当的成本下组装翻译组参考序列，以便在翻译组和蛋白质组水平上进行全局分子研究。通过分析东海海草在富磷、缺磷和恢复磷培养基中的翻译组和蛋白质组，我们发现在富磷条件下，能量和物质生产途径大量上调，使翻译自激活，这是其在HABs中呈指数增长的关键。为了打破自激活，我们证明了使用极低浓度的环己亚胺进行轻微的翻译延迟可以有效地控制开花，而不会伤害其他水生生物和人类。我们的研究结果为控制赤潮提供了新的线索，并证明了RNC-seq是研究具有大基因组和未知基因组的生物体功能的一种经济策略。
Harmful algal blooms (HABs) are symptomatic of ecosystem imbalance, leading to major worldwide marine natural disasters, and seriously threaten the human health. Some HAB algae's exceptional genome size prohibited the genomic investigations on molecular mechanisms, for example, Prorocentrum. This study performed translatome sequencing (RNC-seq) for Prorocentrum donghaiense to assemble the translatome reference sequences on appropriate cost to enable the global molecular study at translatome and proteome levels. By analyzing the translatome and proteome of P. donghaiense in phosphor-rich, phosphor-deficient, and phosphor-restored media, we found massive up-regulation of energy and material production pathways in phosphor-rich conditions that enables autoactivation of translation, which is the key to its exponential growth in HABs. To break down the autoactivation, we demonstrated that mild translation delay using very low concentrations of cycloheximide efficiently controls the blooming without harming other aquatic organisms and humans. Our result provides a novel hint for controlling HABs and demonstrated the RNC-seq as an economic strategy on investigating functions of organisms with large and unknown genomes.