LEVELS AND ROLE OF SUCROSE SYNTHASE, SUCROSE-PHOSPHATE SYNTHASE, AND ACID INVERTASE IN SUCROSE ACCUMULATION IN FRUIT OF ASIAN PEAR
LEVELS AND ROLE OF SUCROSE SYNTHASE, SUCROSE-PHOSPHATE SYNTHASE, AND ACID INVERTASE IN SUCROSE ACCUMULATION IN FRUIT OF ASIAN PEAR
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DOI：
10.21273/jashs.117.2.274
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发表时间：
1992-03-01
影响因子：
1.9
通讯作者：
YAMAKI, S
中科院分区：
文献类型：
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作者：
MORIGUCHI, T;ABE, K;YAMAKI, S
分析了亚洲梨果实果皮中可溶性糖含量和蔗糖代谢酶蔗糖合成酶（SS）（EC 2.4.1.13）、蔗糖磷酸合成酶（SPS）（EC 2.4.1.14）和酸性转化酶（EC 2.4.1.26）的活性，以确定亚洲梨果实中蔗糖积累的关键酶。 日本梨'Chojuro' [Pyrus pyrifolia（Burm. f.）根据成熟果实的分析，Nakai]属于高蔗糖积累型，而中国鸭梨'鸭梨'（P.bretschneideri）属于高蔗糖积累型。在各发育阶段均为低蔗糖积累型。 '长十郎'的SS和SPS活性在成熟过程中随着蔗糖的积累而增加，而'鸭梨'的这些酶的活性在成熟过程中没有增加。 成熟期前者的SS和SPS活性分别是后者的7倍和4倍。 在23个梨品种中，蔗糖分与SS活性密切相关，而SPS活性相关性较弱。 ‘长十郎’和‘鸭梨’可溶性酸性转化酶活性随果实成熟而降低，但与蔗糖含量的关系不显著。 结果表明，SS和SPS是亚洲梨果实蔗糖积累的重要决定因素，可溶性酸性转化酶活性的降低不是蔗糖积累的绝对必要条件。
Soluble sugar content and activities of the sucrose-metabolizing enzymes sucrose synthase (SS) (EC 2.4.1.13), sucrose-phosphate synthase (SPS) (EC 2.4.1.14), and acid invertase (EC 2.4.1.26) were analyzed in the pericarp of fruit from pear cultivars that differed in their potential to accumulate sucrose to identify key enzymes involved in sucrose accumulation in Asian pears. The Japanese pear 'Chojuro' [Pyrus pyrifolia (Burm. f.) Nakai] was characterized as a high-sucrose-accumulating type based on the analysis of mature fruit, while the Chinese pear 'Yali' (P. bretschneideri Rehd.) was a low-sucrose-accumulating type throughout all developmental stages. The activity of SS and SPS in 'Chojuro' increased during maturation concomitant with sucrose accumulation, whereas the activity of these enzymes in 'Yali' did not increase during maturation. The activity of SS and SPS in the former were seven and four times, respectively, higher than those in the latter at the mature stage. Further, among 23 pear cultivars, SS activity was closely correlated with sucrose content, while SPS activity was weakly correlated. Soluble acid invertase activity in 'Chojuro' and 'Yali' decreased with fruit maturation, but the relationships between soluble invertase activity and sucrose content were not significant. The results indicate that SS and SPS are important determinants of sucrose accumulation in Asian pear fruit and that a decrease of soluble acid invertase activity is not absolutely required for sucrose accumulation.