β-cryptoxanthin regulates bone resorption related-cytokine production in human periodontal ligament cells
β-cryptoxanthin regulates bone resorption related-cytokine production in human periodontal ligament cells
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DOI：
10.1016/j.archoralbio.2013.01.005
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发表时间：
2013-07-01
影响因子：
3
通讯作者：
Kanamura, Narisato
中科院分区：
文献类型：
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作者：
Nishigaki, Masaru;Yamamoto, Toshiro;Kanamura, Narisato
目的：隐黄质（β-cry）是一类存在于某些水果和蔬菜中的类胡萝卜素。尽管已经显示β-cry抑制牙槽骨吸收，但其分子机制尚未阐明。在本研究中，我们研究了beta-cry对人牙周膜（hPDL）细胞中骨吸收相关细胞因子产生的影响。设计：hPDL细胞用beta-cry（1 × 10（-7）mol/l）、机械应力（1或6 MPa）和牙龈卟啉单胞菌刺激。用RT-PCR和ELISA分析白细胞介素（IL）-1 β、IL-6、IL-8、肿瘤坏死因子（TNF）-α、骨保护素（OPG）和核因子κ B受体激活因子配体（RANKL）的产生。在用β-cry刺激后，hPDL细胞中没有诱导IL-1 β、IL-6、IL-8和TNF-α的产生，尽管这些细胞因子是在用牙龈卟啉单胞菌刺激后产生的。另一方面，IL-6和IL-8在暴露于6 MPa的机械应力后产生。通过加入β-cry，IL-6和IL-8的产生显著降低。结论：beta-cry可抑制机械应力和牙周致病菌诱导的hPDL细胞产生IL-6和IL-8。此外，β-cry上调OPG的产生。这些结果表明，β-cry可以防止牙周炎的骨吸收。(C)2013爱思唯尔有限公司保留所有权利。
Objective: E-cryptoxanthin (beta-cry) is a type of carotenoid found in certain fruits and vegetables. Although it has been shown that beta-cry inhibits alveolar bone resorption, the molecular mechanisms for this have not yet been clarified. In the present study, we investigated the effects of beta-cry on bone resorption related-cytokine production in human periodontal ligament (hPDL) cells.Design: hPDL cells were stimulated with beta-cry (1 x 10(-7) mol/l), mechanical stress (1 or 6 MPa), and P. gingivalis. The production of interleukin (IL)-1 beta, IL-6, IL-8, tumour necrosis factor (TNF)-alpha, osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-B ligand (RANKL) were analyzed by RT-PCR and ELISA.Results: The production of IL-1 beta, IL-6, IL-8, and TNF-alpha was not induced in hPDL cells after stimulation with beta-cry, although these cytokines were produced after stimulation with P. gingivalis. On the other hand, IL-6 and 1L-8 were produced after exposure to 6 MPa of mechanical stress. The production of IL-6 and IL-8 was significantly decreased by the addition of beta-cry. Furthermore, beta-cry up-regulated the production of OPG, but not RANKL.Conclusion: beta-cry inhibited the production of IL-6 and IL-8 induced by mechanical stress and periodontopathogenic bacteria in hPDL cells. Moreover, beta-cry up-regulated OPG production. These results suggest that beta-cry may prevent bone resorption in periodontitis. (C) 2013 Elsevier Ltd. All rights reserved.