Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain
Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain
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DOI：
10.1051/vetres:2003036
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发表时间：
2003-11-01
影响因子：
4.4
通讯作者：
Zientara, S
中科院分区：
文献类型：
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作者：
Bréard, E;Sailleau, C;Zientara, S
蓝舌病血清型2 （BTV 2）于2000年2月首次在突尼斯得到确认，此后向北和向西传播，感染了其他几个国家和岛屿，包括2000年10月报告出现临床疾病的科西嘉岛。2001年7月，在针对BTV2的疫苗接种运动大约六个月后，岛上再次报告了BT。通过比较2000年和2001年科西嘉BTV - 2野生型病毒分离株和BTV - 2减毒疫苗的基因组2、7和10段序列，并与基因组数据库中已发表的序列进行比较，确定了它们之间的分子关系。科西嘉BTV 2分离株遗传完全稳定。从野生型和疫苗株中获得的第10片段的核苷酸序列存在一定的差异。基于这些差异，选择了可用于RT-PCR区分野生型和疫苗型病毒的引物。
Bluetongue (BT) virus serotype 2 (BTV 2) was first confirmed in Tunisia in February 2000 and has since spread northward and westward, infecting several other countries and islands, including Corsica, where clinical disease was reported in October 2000. BT was again reported on the Island in July 2001, some six months after a vaccination campaign against BTV2. The molecular relationship between isolates of the BTV 2 Corsican wild-type viruses from 2000 and 2001, and the attenuated BTV 2 vaccine were determined by comparing corresponding sequences of genome segments 2, 7 and 10 with each other and with already published sequences available in the genome database. Complete genetic stability was observed between the isolates of the Corsican BTV 2. There was some divergence between the nucleotide sequences of segment 10 obtained from the wild-type and vaccine virus strains. Based on these differences, primers were selected that could be used in RT-PCR to differentiate between the wild-type and the vaccine viruses.