An in vivo model to investigate lymphocyte-mediated immunity during acute hemoparasitic infections. Use of a monoclonal antibody to selectively deplete CD4+ T lymphocytes from thymectomized calves.
An in vivo model to investigate lymphocyte-mediated immunity during acute hemoparasitic infections. Use of a monoclonal antibody to selectively deplete CD4+ T lymphocytes from thymectomized calves.
复制标题
研究急性血液寄生虫感染期间淋巴细胞介导的免疫的体内模型。
DOI：
10.1111/j.1749-6632.2000.tb05294.x
复制
发表时间：
2000
影响因子：
5.2
通讯作者：
Knowles,DP
中科院分区：
文献类型：
--
作者：
Valdez,RA;McGuire,TC;Brown,WC;Davis,WC;Knowles,DP
翻译后摘要：胸腺切除小牛选择性耗尽的CD 4 +T淋巴细胞与特异性的牛CD 4单体（ILA-11）的单克隆抗体（mAb）。在研究的第一周期间，用高负荷剂量的ILA-11处理小牛，然后用随后的较低维持剂量处理小牛。每周通过流式细胞术分析淋巴结和脾活检的PBMC和单核细胞来评估CD 4 +T淋巴细胞的消耗。用高剂量的ILA-11治疗导致来自外周血、外周淋巴结和脾脏的CD 4 +T淋巴细胞的快速和显著的消耗。尽管在给药后第21天，CD 4 +T淋巴细胞缓慢返回外周血、外周淋巴结和脾脏，但在研究期间，耗竭小牛中的CD 4 +T淋巴细胞数量仍低于耗竭前水平。用mAb ILA-11处理的未切除胸腺的小牛未能有效清除CD 4 +T淋巴细胞。在切除胸腺的小牛中开发T淋巴细胞耗竭模型将允许检验牛中需要CD 4 +T淋巴细胞和IFN-γ来控制急性无形体病的假设。在后续计划的研究中，将对缺乏CD 4 +T淋巴细胞的胸腺切除小牛进行实验性感染A。耗竭和非耗竭小牛之间的边缘和疾病参数比较。
Abstract:Thymectomized calves were selectively depleted of CD4+T lymphocytes with a monoclonal antibody (mAb) specific for the bovine CD4 monomer (ILA‐11). Calves were treated with high loading doses of ILA‐11 during the first week of the study then treated with subsequent lower maintenance doses. Depletion of CD4+T lymphocytes was assessed weekly by flow cytometric analysis of PBMC and mononuclear cells from lymph node and spleen biopsies. Treatment with high doses of ILA‐11 resulted in rapid and marked depletion of CD4+T lymphocytes from the peripheral blood, peripheral lymph nodes, and spleen. Although CD4+T lymphocytes slowly returned to the peripheral blood, peripheral lymph nodes, and spleen by day 21 post‐treatment, the numbers of CD4+T lymphocytes in depleted calves remained below pre‐depletion levels for the duration of the study. CD4+T lymphocytes failed to be effectively depleted from a non‐thymectomized calf treated with the mAb ILA‐11. Development of a T lymphocyte depletion model in thymectomized calves will permit testing of the hypothesis that CD4+T lymphocytes and IFN‐γ are required in cattle for control of acute anaplasmosis. In subsequent planned studies, thymectomized calves depleted of CD4+T lymphocytes will be experimentally infected with A. marginale and parameters of disease compared between depleted and non‐depleted calves.