Involvement of SigT and RstA in the differentiation of Streptomyces coelicolor
Involvement of SigT and RstA in the differentiation of Streptomyces coelicolor
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SigT和RstA参与天蓝色链霉菌的分化
DOI：
10.1016/j.febslet.2009.09.025
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发表时间：
2009-10-06
期刊：
影响因子：
3.5
通讯作者：
Li, Yong-Quan
中科院分区：
文献类型：
--
作者：
Mao, Xu-Ming;Zhou, Zhan;Li, Yong-Quan
SigT是天蓝色链霉菌中的ECF σ因子。sigT与其推定的抗σ因子基因rstA位于一个推定的操纵子中，并且SigT可以与RstA物理相互作用。sigT或rstA的缺失导致形态发育加速和抗生素的产生增强，伴随着chpE，chpH，actII-orf 4和redD的过表达。此外，在rstA丧失后，无法检测到SigT。这些数据表明，SigT对分化具有负面作用，并且RstA通过推定的拮抗机制和在转录后水平负调节SigT活性。（uniprotkb：Q9 S6 U2）与sigT发生物理相互作用（MI：0915）（uniprotkb：O 86856）通过下拉（MI：0096）MINT-7262539：rpoC（uniprotkb：Q8 CJT 1）与sigT发生物理相互作用（MI：0915）（uniprotkb：O 86856）通过下拉（MI：0096）MINT-7262574：RstA（uniprotkb：Q9 S6 U2）通过抗标签共免疫沉淀（MI：0007）与sigT（uniprotkb：086856）物理相互作用（MI：0915）（C）2009欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
SigT is an ECF sigma factor in Streptomyces coelicolor. sigT and its putative anti-sigma factor gene rstA are located in one putative operon, and SigT could physically interact with RstA. Deletion of sigT or rstA caused accelerated morphological development and enhanced production of antibiotics, concomitant with over-expression of chpE, chpH, actII-orf4 and redD. Furthermore, SigT was undetectable after loss of rstA. These data suggested that SigT has a negative role on differentiation and that RstA negatively regulates the SigT activity through a putative antagonistic mechanism and at the post-transcriptional level.Structured summary:MINT-7262599, MINT-7262614:RstA (uniprotkb:Q9S6U2) physically interacts (MI:0915) with sigT (uniprotkb: O86856) by pull down (MI:0096) MINT-7262539:rpoC (uniprotkb:Q8CJT1) physically interacts (MI:0915) with sigT (uniprotkb:O86856) by pull down (MI:0096) MINT-7262574:RstA (uniprotkb:Q9S6U2) physically interacts (MI:0915) with sigT (uniprotkb:O86856) by anti tag coimmunoprecipitation (MI:0007) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.