Efficient Cultivation Conditions for Human Limbal Epithelial Cells
Efficient Cultivation Conditions for Human Limbal Epithelial Cells
复制标题
人角膜缘上皮细胞的高效培养条件
DOI：
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发表时间：
2008
影响因子：
4.5
通讯作者：
Y. Son
中科院分区：
文献类型：
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作者：
Mee;Jae Lim Lee;J. Oh;M. Shin;K. Shin;W. Wee;J. Lee;Ki;Y. Son
为了比较角膜缘上皮细胞在不同培养条件下的干生态位，将悬浮的人角膜缘上皮细胞（HLEC）接种在3T3预处理的平板上，将其他悬浮细胞铺在冷冻保存或冻干的羊膜（AM）上。全部培养10至12天。在培养的 HLEC 中对 ATP 结合盒、G 亚家族成员 2 (ABCG2)、p63、细胞角蛋白 12 和连接蛋白 43 进行逆转录聚合酶链反应 (RT-PCR)，并比较它们的表达水平。所有检查标记物的 mRNA 表达显示，冷冻保存和冻干 AM 细胞之间没有统计学上的显着差异。 RT-PCR和免疫荧光染色显示，AMs上培养的细胞中p63和细胞角蛋白12的表达显着低于3T3共培养的细胞。无论 AM 的保存方法如何，在 AM 上培养的 HLEC 都显示出增殖和分化减少，同时保持茎特性。
To compare the stem niche in different culture conditions of limbal epithelial cells, the suspended human limbal epithelial cells (HLECs) were seeded on the 3T3-pretreated plates and the other suspended cells were plated on amniotic membranes (AMs) which were either cryo-preserved or freeze-dried. All were cultured for 10 to 12 days. Reverse transcription-polymerase chain reaction (RT-PCR) for ATP-binding casette, subfamily G, member 2 (ABCG2), p63, cytokeratin 12, and connexin 43 were performed in cultivated HLECs and their expression levels were compared. The mRNA expression of all markers examined showed no statistically significant differences between the cells on cryo-preserved and on freeze-dried AM. The expression of p63 and cytokeratin 12 in cultivated cells on AMs were significantly lower than those in 3T3-cocultured cells on RT-PCR and immunofluorescent staining. Cultivated HLECs on AMs showed reduced proliferation and differentiation while maintaining stem-property regardless of the preservative method of AM.
羊膜培养人角膜缘细胞对兔角膜上皮的功能重建。
影响因子：
2.2
作者：
Du,Yiqin;Chen,Jing;Funderburgh,JamesL;Zhu,Xiuan;Li,Lingsong
通讯作者：
Li,Lingsong
角膜缘到角膜缘清创后的上皮再生。
影响因子：
4.4
作者：
Chung,EH;DeGregorio,PG;Wasson,M;Zieske,JD
通讯作者：
Zieske,JD