Prevalence of human papillomavirus in archival samples obtained from patients with cervical pre-malignant and malignant lesions from Northeast Brazil.
Prevalence of human papillomavirus in archival samples obtained from patients with cervical pre-malignant and malignant lesions from Northeast Brazil.
复制标题
DOI：
10.1186/1756-0500-3-96
复制
发表时间：
2010-04-08
影响因子：
1.8
通讯作者：
Villa LL
中科院分区：
文献类型：
--
作者：
Fernandes JV;Meissner RV;Carvalho MG;Fernandes TA;Azevedo PR;Sobrinho JS;Prado JC;Villa LL
人乳头瘤病毒（HPV）被认为是宫颈癌的必要但不充分的原因。在这项研究中，我们的目的是评估一系列癌前病变和恶性宫颈病变病例中HPV的患病率，确定病毒基因型，并根据病变类型，年龄范围和其他考虑的变量评估其分布模式。将样本提交组织病理学修正检查，并通过聚合酶链反应（PCR）分析HPV DNA的存在，然后通过斑点杂交进行HPV分型。在分析的样本中，53.7%显示癌前宫颈病变，46.3%出现宫颈癌。大多数癌症样本（84.1%）被归类为浸润性癌。这些癌症患者的平均年龄为47.3岁。癌前病变患者HPV感染率为82.4%，癌症患者为92.0%。HPV 16是最流行的类型，其次是HPV 18和58，包括单次和双重感染。在11.6%的样本中检测到双重感染，最常见的组合是HPV 16+18。与巴西其他地区的情况相比，在研究地区，宫颈癌似乎发生在较低年龄段的妇女中。此外，在CIN 3和癌症患者中，我们观察到已婚妇女、有不止一个性伴侣的妇女、吸烟者和受教育程度低于小学的人的比例高于他们的同行。在巴西东北部的宫颈癌患者中，癌前病变患者的HPV总患病率为82.4%，宫颈癌患者的HPV总患病率为92.0%。HPV 16是最常见的类型，其次是HPV 18和58。最常见的双重感染是HPV 16+18。宫颈癌似乎发生在巴西东北部较低年龄段的妇女中。在CIN 3和癌症患者中，我们观察到已婚妇女、有一个性伴侣以上的妇女、吸烟者和受教育程度低于小学的人的比例高于他们的同行。
Human Papillomavirus (HPV) is considered as a necessary, but not sufficient, cause of cervical cancer. In this study, we aimed to assess the prevalence of HPV in a series of pre-malignant and malignant cervical lesion cases, to identify the virus genotypes, and to assess their distribution pattern according to lesion type, age range, and other considered variables. The samples were submitted to histopathological revision examination and analysed by polymerase chain reaction (PCR) for the presence of HPV DNA, followed by HPV typing by dot blot hybridisation. Of the analysed samples, 53.7% showed pre-malignant cervical lesions, and 46.3% presented with cervical cancer. Most cancer samples (84.1%) were classified as invasive carcinoma. The mean age of these cancer patients was 47.3 years. The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cancer patients. HPV 16 was the most prevalent type, followed by HPV 18 and 58, including both single and double infections. Double infection was detected in 11.6% of the samples, and the most common combination was HPV 16+18. Cervical cancer appears to occur in women in a lower age range in the studied area, compared to the situation in other Brazilian regions. Furthermore, among the patients with CIN 3 and those with cancer, we observed a higher proportion of married women, women with more than one sexual partner, smokers, and individuals with less than an elementary education, relative to their counterparts. The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cervical cancer patients from Northeast Brazil. HPV 16 was the most prevalent type, followed by HPV 18 and 58. The most common double infection was HPV 16+18. Cervical cancer appears to occur in women in a lower age range in the Northeast Brazil. Among the patients with CIN 3 and those with cancer, we observed a higher proportion of married women, women with more than one sexual partner, smokers, and individuals with less than an elementary education, relative to their counterparts.