Deep sequencing of the microRNA expression in fall dormant and non-dormant alfalfa.
Deep sequencing of the microRNA expression in fall dormant and non-dormant alfalfa.
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秋季休眠和非休眠苜蓿中 microRNA 表达的深度测序
DOI：
10.1016/j.gdata.2014.09.007
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Fan W;Shi P;Wang C
MicroRNAs （miRNAs）在转录后基因调控中发挥关键作用，通过mRNA降解或翻译抑制下调靶基因。越来越多的证据表明它们在植物发育过程中起着至关重要的作用。通过高通量测序在全球基因组水平上鉴定mirna对植物mirna的功能表征至关重要。苜蓿（Medicago sativa L.）是世界上种植最广泛的多年生饲草豆科植物之一。秋季休眠是苜蓿的一种适应性性状，关系到苜蓿的生物量生产和冬季生存。然而，目前对mirna介导的紫花苜蓿秋季休眠发育调控知之甚少。在这里，我们在研究中提供了详细的实验方法和分析管道来鉴定对秋季休眠有响应的miRNAs （Fan W et al.，不同休眠Medicago sativa L. microRNAs在秋季休眠中的基因组范围鉴定，已提交发表），以进行可重复性研究。我们的工作中产生的数据为理解mirna在苜蓿季节变化和生长调节中的作用提供了有意义的信息。
MicroRNAs (miRNAs) play a critical role in post-transcriptional gene regulation that down-regulates target genes by mRNA degradation or translational repression. Evidence is increasing for their crucial roles during plant development. Identification of miRNAs at the global genome-level by high-throughput sequencing is essential to functionally characterize miRNAs in plants. Alfalfa (Medicago sativa L.) is one of the most widely cultivated perennial forage legumes worldwide. Fall dormancy is an adaptive character related to the biomass production and winter survival in alfalfa. However, little is known about miRNA-mediated developmental regulation of fall dormancy in alfalfa. Here, we provide detailed experimental methods and analysis pipeline in our study to identify miRNAs that were responsive to fall dormancy (Fan W et al., Genome-wide identification of different dormant Medicago sativa L. microRNAs in response to fall dormancy, submitted for publication) for reproducible research. The data generated in our work provide meaningful information for understanding the roles of miRNAs in response to seasonal change and growth regulation in alfalfa.