Fluorescence biosensing system with a UV-LED excitation for L-leucine detection
Fluorescence biosensing system with a UV-LED excitation for L-leucine detection
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DOI：
10.1016/j.snb.2010.01.059
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发表时间：
2010-04-08
影响因子：
8.4
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
--
作者：
Koshida, Tomoyuki;Arakawa, Takahiro;Mitsubayashi, Kohji
利用高强度紫外发光二极管（UV-LED）激发系统（λ = 340 nm）、光纤探针和光电倍增管，研制了一种高灵敏度的L-亮氨酸生物传感器。生物传感器是通过将固定L-亮氨酸脱氢酶的聚四氟乙烯（PTFE）膜与2-甲基丙烯酰氧乙基磷酰胆碱聚合物（PMEH）聚合物连接到光纤上制成的。通过将传感器探针浸入含有烟酰胺腺嘌呤二核苷酸（NAD（+））溶液的磷酸盐缓冲液中来测量水溶液中的L-亮氨酸浓度。将NAD（+）与L-亮氨酸溶液反应产生的烟酰胺腺嘌呤二核苷酸（NADH）荧光信号导入光电倍增管和信号检测器。荧光强度与L-亮氨酸浓度在5 μ M ~ 10 mM范围内呈线性关系。校准范围可用于枫糖浆尿病（MSUD）测定的临床应用。UV-LED生物传感系统的高灵敏度和低功耗有助于提高MSUD患者日常自我护理的生活质量。(c)2010 Elsevier B. V.保留所有权利。
A high-sensitive L-leucine biosensors consisting of L-Ieucine dehydrogenase (LeuDH) immobilized membrane on the edge of a fiber was developed and demonstrated using a high-intensity ultra violet light-emitting diodes (UV-LED) excitation system (lambda = 340 nm), an optical fiber probe and a photomultiplier tube. The biosensors were fabricated by attaching L-leucine dehydrogenase immobilized polytetrafluoroethylene (PTFE) membrane with 2-methacryloyloxyethyl phosphorylcholine polymer (PMEH) polymer to the optical fiber. A L-leucine concentration in an aqueous solution was measured by immersing the sensor probes in the phosphate buffer which contained nicotinamide adenine dinucleotide (NAD(+)) solution. The fluorescent signals of nicotinamide adenine dinucleotide (NADH), produced by enzymatic reactions between NAD(+) and an installation of L-leucine solution, were then guided to the photomultiplier tube and a signal detector. The fluorescent intensities were related linearly to the L-Ieucine concentration from 5 mu M to 10 mM. The calibration ranges were acceptable for clinical applications of maple syrup urine disease (MSUD) determinations. High-sensitive and low power consumption of a UV-LED biosensing system allow to be contributed to their enhance quality of life for daily self-care of MSUD patients. (c) 2010 Elsevier B.V. All rights reserved.