Probing and modulating the interactions of the DNAzyme with DNA-functionalized nanoparticles
Probing and modulating the interactions of the DNAzyme with DNA-functionalized nanoparticles
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探测和调节 DNAzyme 与 DNA 功能化纳米粒子的相互作用
DOI：
10.1016/j.cclet.2021.09.039
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发表时间：
2022-05-20
影响因子：
9.1
通讯作者：
Wu, Tongbo
中科院分区：
文献类型：
--
作者：
Hu, Yuqiang;Zhang, Zhen;Wu, Tongbo
DNA功能化的金纳米粒子是生物医学和临床诊断中最通用的生物纳米材料之一。在此，我们发现DNA酶切割底物的性能与其长度高度相关。这种有趣的现象只出现在DNA功能化的金纳米粒子的界面。我们系统地研究了这一现象的原因。我们证实，DNA酶与延伸的核苷酸不匹配其底物链是脆弱的非特异性吸附，静电排斥，和空间位阻。基于我们对这一现象的进一步理解，我们成功地开发了一种高灵敏度和特异性的生物传感器来检测人脱嘌呤/脱嘧啶核酸内切酶1。(C)2021由Elsevier B. V.代表中国化学会和中国医学科学院药物研究所出版。
DNA-functionalized gold nanoparticles are one of the most versatile bionanomaterials for biomedical and clinical diagnosis. Herein, we discovered that the performance of DNAzyme cleaving the substrate is highly related to its length. This intriguing phenomenon only appears at the interfaces of DNA-functionalized gold nanoparticles. We systematically investigated the causes of this phenomenon. We conjectured that the DNAzyme with extended nucleotides that do not match its substrate strand is vulnerable to non-specific adsorption, electrostatic repulsion, and steric hindrance. Based on our improved understanding of this phenomenon, we have successfully developed a highly sensitive and specific amplifiable biosensor to detect human apurinic/apyrimidinic endonuclease 1. (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.