AGROINFECTION, AN ALTERNATIVE ROUTE FOR VIRAL-INFECTION OF PLANTS BY USING THE TI PLASMID
AGROINFECTION, AN ALTERNATIVE ROUTE FOR VIRAL-INFECTION OF PLANTS BY USING THE TI PLASMID
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DOI：
10.1073/pnas.83.10.3282
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发表时间：
1986-05-01
影响因子：
11.1
通讯作者：
WALDEN, R
中科院分区：
文献类型：
--
作者：
GRIMSLEY, N;HOHN, B;WALDEN, R
大多数植物病毒是通过昆虫媒介传播的。我们提出了一种引入传染性病毒DNA的替代方法，该方法利用农杆菌将DNA从细菌细胞转移到植物的能力。选择花椰菜花叶病毒作为最具特征的植物DNA病毒，可以通过蚜虫、病毒颗粒、病毒DNA或适当处理的克隆DNA导入植物。我们发现，萝卜的系统侵染是由根癌农杆菌的伤害和接种引起的，根癌农杆菌中有多个花椰菜花叶病毒基因组被插入到诱导肿瘤的质粒的T-DNA中。因此，这种结构允许病毒基因组一旦进入植物就从T-DNA中逃逸出来。肿瘤诱导质粒和病毒DNA的结合使用为分子生物学方法开辟了道路，而这两个系统单独使用都是不可能的。
Most plant viruses are transmitted by insect vectors. We present an alternative method for the introduction of infectious viral DNA that uses the ability of Agrobacterium to transfer DNA from bacterial cells to plants. Cauliflower mosaic virus was chosen to develop this method because it is the best characterized plant DNA virus and can be introduced into plants via aphids, virus particles, viral DNA, or suitably treated cloned DNA. We show that systemic infection of turnips results from wounding and inoculation with strains of Agrobacterium tumefaciens in which more than one genome of cauliflower mosaic virus have been placed tandemly in the T-DNA of the tumor-inducing plasmid. Thus such constructions allow escape of the viral genome from the T-DNA once inside the plants. The combined use of the tumor-inducing plasmid and viral DNA opens the way to molecular biological approaches that are not possible with either system alone.