Isolation of 1,796 SSR clones from SSR-enriched DNA libraries of bunching onion (Allium fistulosum)
Isolation of 1,796 SSR clones from SSR-enriched DNA libraries of bunching onion (Allium fistulosum)
复制标题
DOI：
10.1007/s10681-007-9398-2
复制
发表时间：
2007-03
期刊：
影响因子：
1.9
通讯作者：
H. Tsukazaki;T. Nunome;H. Fukuoka;H. Kanamori;I. Kono;K. Yamashita;T. Wako;A. Kojima
中科院分区：
文献类型：
--
作者：
H. Tsukazaki;T. Nunome;H. Fukuoka;H. Kanamori;I. Kono;K. Yamashita;T. Wako;A. Kojima
洋葱（Allium fistulosumL.）是日本最重要的蔬菜之一。为建立洋葱分子育种的遗传基础，通过对富集SSR的基因组DNA文库进行大规模测序，获得了1,796个SSR克隆。其中1,331个（74.1%）含有（GT）n重复序列（n> 5），314个（17.5%）含有（GA）n重复序列。在含（GT）n和（GA）n的克隆中，SSR重复数平均分别为10.5和10.4。以5个葱头自交系为样本，在所研究的100个SSR位点中平均检测到3.2个等位基因，多态信息含量平均为0.55。这些结果表明，洋葱SSR标记是非常丰富的高信息量的遗传标记。
Bunching onion (Allium fistulosumL.) is one of the most important vegetables in Japan. To establish a genetic basis for molecular breeding of bunching onion, we isolated 1,796 simple sequence repeat (SSR) clones by large-scale sequencing of SSR-enriched genomic DNA libraries. Of these, 1,331 (74.1%) contained (GT)nrepeats (n> 5), while 314 (17.5%) were (GA)n-containing clones. The average number of SSR repeats was 10.5 and 10.4 in the (GT)n- and (GA)n-containing clones, respectively. In a sample of five bunching onion inbred lines, an average of 3.2 alleles were detected in the 100 SSR loci investigated, with the polymorphic information content averaging 0.55. These results indicate that bunching onion SSRs are very rich sources of highly informative genetic markers.