Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain.
Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain.
复制标题
小鼠蛋白磷酸酶 ppm1l 基因的靶向破坏会导致大脑结构异常。
DOI：
10.1002/1873-3468.12429
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发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Kusano;R.;Kousuke Fujita;K.;Yasuharu Shinoda;Y.;Nagaura;Y.;Kiyonari;H.;Abe;T;Watanabe;T.;Matsui;Y.;Fukaya;M.;Sakagami;H.;Sato;T.;Funahashi;J.-I.;Ohnishi;M.;Tamura;S.;Kobayashi;T.
PPM1L是金属依赖蛋白磷酸酶(PPM)家族的一员，参与调节应激激活蛋白激酶途径和神经酰胺运输。然而，PPM1L在大脑中的生理功能尚不清楚。在这项研究中，我们建立并分析了ppm1l缺陷小鼠，以研究PPM1L在大脑中的功能。我们的结果表明，在小鼠发育过程中，ppm1在中枢神经系统中高表达，ppm1Δ/Δ小鼠表现出运动能力受损和前脑的形态异常。电子显微镜和免疫组织化学分析表明，这些异常是由于轴索形成受损所致。我们的新发现表明PPM1L在大脑发育中起着重要作用。
PPM1L, a member of the metal‐dependent protein phosphatase (PPM) family, is involved in regulating the stress‐activated protein kinase pathway and ceramide trafficking. However, the physiological function of PPM1L in the brain is unclear. In this study, we generated and analyzedppm1l‐deficient mice in order to investigate PPM1L functions in the brain. Our results indicate thatppm1lis highly expressed in the central nervous system during mouse development and thatppm1lΔ/Δmice display impaired motor performance and morphological abnormalities in the forebrain. Electron microscopic and immunohistochemical analyses suggest that these abnormalities are due to impaired axonal tract formation. Our novel findings suggest an important role for PPM1L in brain development.