Effect of endogenous prostaglandins on acetylcholine release from dog trachealis muscle.
Effect of endogenous prostaglandins on acetylcholine release from dog trachealis muscle.
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内源性前列腺素对狗气管肌乙酰胆碱释放的影响。
DOI：
10.1152/jappl.1987.62.5.1837
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发表时间：
1987
影响因子：
3.3
通讯作者：
J. Martin
中科院分区：
文献类型：
--
作者：
S. Shore;B. Collier;J. Martin
我们使用放射性酶技术来测量前列腺素合成抑制剂吲哚美辛以及外源性前列腺素 E2 (PGE2) 和前列腺素 I2 (PGI2) 在持续电场刺激（EFS；2 Hz、2 ms 脉冲持续时间、50 V 15 分钟）过程中对犬气管平滑肌 (TSM) 乙酰胆碱 (ACh) 流出的影响。来自吲哚美辛（INDO，10（-6）M）预处理和对照TSM的ACh流出随着连续刺激而增加。然而，INDO 处理的肌肉中 ACh 的流出量大于对照肌肉。 INDO 增加了 TSM 应对 EFS 所产生的紧张感。在 EFS 三个阶段的第一个阶段，PGE2 (10(-8) M) 和 PGI2 (10(-6) M) 对 INDO 预处理的 TSM 的 ACh 流出没有任何影响。然而，PGI2 和 PGE2 阻止了在后续刺激中观察到的乙酰胆碱流出的逐渐增加。 PGE2 但不是 PGI2 减少 EFS 引起的 TSM 收缩。我们的结果表明，内源性前列腺素（可能是 PGE2）确实在体外抑制犬 TSM 中 EFS 诱发的 ACh 释放，但表明这些前列腺素主要通过突触后机制调节 EFS 诱发的收缩。
We used a radioenzymatic technique to measure effects of the prostaglandin synthesis inhibitor indomethacin and of exogenous prostaglandin E2 (PGE2) and prostaglandin I2 (PGI2) on acetylcholine (ACh) efflux from canine tracheal smooth muscle (TSM) during sustained electrical field stimulation (EFS; 2 Hz, 2 ms pulse duration, 50 V for 15 min). ACh efflux from indomethacin (INDO, 10(-6) M)-pretreated and control TSM increased with consecutive stimulations. However, efflux of ACh was greater in INDO-treated than control muscles. INDO increased the tension produced by TSM in response to EFS. Neither PGE2 (10(-8) M) nor PGI2 (10(-6) M) had any effect on ACh efflux from INDO-pretreated TSM during the first of three periods of EFS. However, PGI2 and PGE2 prevented the progressive increase in ACh efflux observed on subsequent stimulations. PGE2 but not PGI2 decreased contractions of TSM caused by EFS. Our results demonstrate that endogenous prostaglandins, probably PGE2, do inhibit EFS-evoked ACh release from canine TSM in vitro, but suggest that these prostaglandins modulate EFS-evoked contractions predominantly by postsynaptic mechanisms.