Synergistic Effects of Schwann- and Muscle-Derived Factors on Motoneuron Survival Involve GDNF and Cardiotrophin-1 (CT-1)
Synergistic Effects of Schwann- and Muscle-Derived Factors on Motoneuron Survival Involve GDNF and Cardiotrophin-1 (CT-1)
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雪旺和肌肉源性因子对运动神经元存活的协同作用涉及 GDNF 和心肌营养蛋白-1 (CT-1)
DOI：
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发表时间：
1998
影响因子：
5.3
通讯作者：
O. Delapeyrière
中科院分区：
文献类型：
--
作者：
V. Arce;R. A. Pollock;J. Philippe;D. Pennica;C. Henderson;O. Delapeyrière
中枢神经元的存活依赖于不同细胞类型产生的多种神经营养因子。我们证明了肌肉和雪旺细胞系条件培养液对纯化的胚胎14.5天大鼠运动神经元的存活有很强的协同作用。不同的证据表明，胶质细胞源性神经营养因子(GDNF)和心肌营养素-1(CT-1)参与了这种协同作用。它们在运动神经元环境中的表达是分段的：GDNF转录本主要在雪旺细胞系中表达，而ct-1mRNA在肌管中存在。GDNF的封闭抗体可将雪旺细胞系条件培养液的营养活性抑制75%，而CT-1抗体可使肌管来源的活性降低46%。CT-1和GDNF协同作用增强运动神经元在体外的存活。在体内，单个运动神经元共表达GDNF和CT-1受体成分。因此，GDNF和CT-1分别是雪旺氏细胞和肌肉细胞提供营养支持的主要成分。它们共同作用于单个运动神经元的可能性表明，在决定是死亡还是生存时，运动神经元必须整合来自不同细胞伙伴的不同信号。
The survival of central neurons depends on multiple neurotrophic factors produced by different cell types. We demonstrate that media conditioned by muscle and Schwann cell lines show strong synergistic effects on survival of purified embryonic day 14.5 rat motoneurons in culture. Different lines of evidence implicate glial cell line-derived neurotrophic factor (GDNF) and cardiotrophin-1 (CT-1) in this synergy. Their expression in the environment of the motoneuron is compartmentalized: gdnf transcripts are expressed principally in Schwann cell lines, whereas ct-1 mRNA is present in myotubes. Blocking antibodies to GDNF inhibit the trophic activity of Schwann cell line-conditioned media by 75%, whereas CT-1 antibodies diminish the myotube-derived activity by 46%. CT-1 and GDNF act synergistically to enhance motoneuron survival in vitro. In vivo, individual motoneurons coexpress both GDNF and CT-1 receptor components. GDNF and CT-1, therefore, are major components of the trophic support provided by the Schwann and muscle cells, respectively. The possibility that they act together on individual motoneurons suggests that the motoneuron must integrate distinct signals from different cellular partners when deciding whether to die or to survive.
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单克隆抗体调节神经生长因子与 PC12 细胞的相互作用。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chandler,CE;Parsons,LM;Hosang,M;Shooter,EM
通讯作者：
Shooter,EM
影响因子：
4.6
作者：
V. Pachnis;B. Mankoo;F. Costantini
通讯作者：
V. Pachnis;B. Mankoo;F. Costantini
影响因子：
11.4
作者：
Jowett, T;Yan, YL
通讯作者：
Yan, YL
Cardiotropin-1 在小鼠心管中早期表达并促进心肌细胞存活。
DOI：
10.1242/dev.122.2.419
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Sheng,Z;Pennica,D;Wood,WI;Chien,KR
通讯作者：
Chien,KR