Neural differentiation of adipose-derived stem cells isolated from GFP transgenic mice
Neural differentiation of adipose-derived stem cells isolated from GFP transgenic mice
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DOI：
10.1016/j.bbrc.2005.05.096
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发表时间：
2005-07-22
影响因子：
3.1
通讯作者：
Suzuki, H
中科院分区：
文献类型：
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作者：
Fujimura, J;Ogawa, R;Suzuki, H
利用绿色荧光蛋白（GFP）转基因小鼠的细胞，我们最近报道了脂肪来源的基质细胞（ASCs）可以在体外分化为间充质谱系。在这项研究中，我们进行了神经诱导使用ASC从GFP转基因小鼠，并能够诱导这些ASC到神经元和神经胶质细胞谱系。大多数神经诱导的细胞在形态上呈双极或多极外观，并表达神经元标记物。电子显微镜显示其神经元形态。一些细胞也显示出胶质细胞表型，如免疫细胞化学所示。本研究清楚地表明，来源于GFP转基因小鼠的ASCs在体外分化为神经谱系，这表明这些细胞可能为进一步的神经干细胞研究提供理想的来源，并可能用于神经系统疾病的治疗应用。(c)2005年爱思唯尔公司All rights reserved.
Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice, we have recently reported that adipose-derived stromal cells (ASCs) could differentiate into mesenchymal lineages in vitro. In this study, we performed neural induction using ASCs from GFP transgenic mice and were able to induce these ASCs into neuronal and glial cell lineages. Most of the neurally induced cells showed bipolar or multipolar appearance morphologically and expressed neuronal markers. Electron microscopy revealed their neuronal morphology. Some cells also showed glial phenotypes, as shown immunocytochemically. The present study clearly shows that ASCs derived from GFP transgenic mice differentiate into neural lineages in vitro, suggesting that these cells might provide an ideal source for further neural stem cell research with possible therapeutic application for neurological disorders. (c) 2005 Elsevier Inc. All rights reserved.