ABNORMAL CENTROSOMES IN COLD-TREATED DROSOPHILA EMBRYOS
ABNORMAL CENTROSOMES IN COLD-TREATED DROSOPHILA EMBRYOS
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DOI：
10.1016/0014-4827(89)90336-4
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发表时间：
1989-10-01
影响因子：
3.7
通讯作者：
MARCHINI, D
中科院分区：
文献类型：
--
作者：
CALLAINI, G;MARCHINI, D
In this study we examine the effect on the centrosomes of cold treatment of early Drosophila embryos. Prolonged cold treatment during the mitotic divisions which lead to the formation of the blastoderm causes arrest at metaphase of the nuclear divisions. When examined with immunofluorescence microscopy in mitotic spindles show marked pole splitting with the formation of supernumerary and irregularly sized centers, all able to nucleate microtubules. In embyros recovered for longer periods the additional organizing centers become ring-shaped and lose their nucleating properties. Cold treatment of embryos during the cellularization of the blastoderm results in marked fragmentation of the centrosomes, but nucleating capacity is preserved. Sometimes the centrioles come away from the pericentriolar material and their structure is seen to be modified.
