OsNF-YC10, a seed preferentially expressed gene regulates grain width by affecting cell proliferation in rice
OsNF-YC10, a seed preferentially expressed gene regulates grain width by affecting cell proliferation in rice
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OsNF-YC10，一种种子优先表达基因，通过影响水稻细胞增殖来调节粒宽
DOI：
10.1016/j.plantsci.2018.09.021
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发表时间：
2019-03-01
期刊：
影响因子：
5.2
通讯作者：
Yao, Jialing
中科院分区：
文献类型：
--
作者：
Jia, Shuzhao;Xiong, Yufei;Yao, Jialing
籽粒大小和形状是决定粮食产量的重要因素。在本研究中，我们克隆并鉴定了编码组蛋白转录因子的核因子-Y转录因子家族成员OsNF-YC10。QRT-PCR和mRNA原位杂交分析表明，OsNF-YC10在发育后期的胚乳和颖壳中高表达。结果表明，OsNF-YC10是一种具有转录激活活性的核蛋白。用CRISPR/Cas9技术生产的osnf-yc10品系表现出窄、细、轻的颗粒。细胞学实验表明，osnf-yc10品系的颖壳细胞数显著低于wt品系。在OsNF-YC10 RNAi转基因株系中观察到一致的窄、细、亮颗粒。此外，细胞数量在粒宽方向上比WT方向减少。这些结果表明OsNF-YC10在决定颗粒大小和形状方面起着重要作用。OsNF-YC10还影响正调控基因GW8、负调控基因GW7和细胞周期调控基因CYCD4、CYCA2.1、CYCB2.1、CYCB2.2、E2F2的表达。综上所述，OsNF-YC10可能通过影响颖壳细胞的增殖来调节籽粒的大小和形状。
Grain size and shape are important factors in determining the grain yield. In this study, OsNF-YC10, a member of the NF-Y transcription factor family encoding a putative histone transcription factor, was cloned and characterized. qRT-PCR and mRNA in situ hybridization analysis revealed that OsNF-YC10 was highly expressed in endosperm and spikelet hull at late developmental stages. The results showed that OsNF-YC10 was a nuclear protein showing transcription activation activity. The osnf-yc10 lines, produced using CRISPR/Cas9 technology, showed narrow, thin and light grains. Cytological experiments revealed significantly reduced cell number of spikelet hull in osnf-yc10 lines compared with that in WT. Narrow, thin, and light grains were found consistently in OsNF-YC10 RNAi transgenic lines. Moreover, the number of cells decreased in the grain-width direction than WT. These results indicated that OsNF-YC10 plays an important role in determining grain size and shape. OsNF-YC10 was further revealed to influence the expression of GW8 (a positive regulator of grain width), GW7 (a negative regulator of grain width) and cell cycle-regulated genes CYCD4, CYCA2.1, CYCB2.1, CYCB2.2, E2F2. Taken together, it is suggested that OsNF-YC10 regulates the grains size and shape by influencing the cell proliferation of spikelet hulls.