Parabiosis Model Does Not Show Presence of Circulating Osteoprogenitor Cells
Parabiosis Model Does Not Show Presence of Circulating Osteoprogenitor Cells
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DOI：
10.1002/dvg.20602
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发表时间：
2010-03-01
期刊：
影响因子：
1.5
通讯作者：
Clark, Stephen H.
中科院分区：
文献类型：
--
作者：
Boban, Ivana;Barisic-Dujmovic, Tatjana;Clark, Stephen H.
本研究的目的是确定外周血中骨祖细胞的存在。用联体共生模型进行实验，其中成骨细胞特异性转基因小鼠（Col2.3GFP或hOC-GFP）与其中疱疹病毒胸苷激酶基因在胶原α 1启动子（Col2.3 Delta TK）控制下的转基因小鼠手术连接。该方法允许通过更昔洛韦（GCV）治疗对成骨细胞进行条件性消融。在用GCV处理15天或1.5-2个月的Parabions中，在来自Col2.3 Delta TK Parabions的组织学制备物或骨髓基质细胞培养物中未检测到GFP（hOC-GFP或Col2.3GFP）表达。最后，将Col2.3GFP/Col2.3 Delta TK对用GCV处理15天，并允许从GCV中恢复3个月。同样，在Co12.3 Delta TK副殖子中未能检测到表达Col2.3GFP的细胞。这些观察结果，至少在该模型系统的限度内，允许得出骨祖细胞不容易循环的结论。创世纪48：171-182，2010。(C)2010 Wiley-Liss，Inc.
The goal of this study was to determine the presence of osteoprogenitor cells in the peripheral blood. Experiments were conducted with a parabiosis model in which osteoblast specific transgenic mice (Col2.3GFP or hOC-GFP) were surgically joined with a transgenic mouse where herpes virus thymidine kinase gene is under the control of the collagen alpha 1 promoter (Col2.3 Delta TK). This method permits conditional ablation of osteoblasts by ganciclovir (GCV) treatment. In parabionts treated with GCV for 15 days or 1.5-2 months, GFP (hOC-GFP or Col2.3GFP) expression was not detected in histological preparations or in marrow stromal cell cultures from the Col2.3 Delta TK parabiont. Finally, Col2.3GFP/Col2.3 Delta TK pairs were treated with GCV for 15 days and allowed to recover from GCV for 3 months. Again there was a failure to detect Col2.3GFP expressing cells in the Co12.3 Delta TK parabiont. These observations, at least within the limits of this model system, allow the conclusion that osteoprogenitor cells do not readily circulate. genesis 48:171-182, 2010. (C) 2010 Wiley-Liss, Inc.