The application of schlieren optics for detection of protein bands and other phenomena in polyacrylamide gel electrophoresis
The application of schlieren optics for detection of protein bands and other phenomena in polyacrylamide gel electrophoresis
复制标题
纹影光学在聚丙烯酰胺凝胶电泳中蛋白质条带等现象检测中的应用
DOI：
10.1002/elps.1150110504
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Hidehiro Kubota
中科院分区：
文献类型：
--
作者：
Toshio Takagi;Hidehiro Kubota
在聚丙烯酰胺凝胶电泳过程中，采用了现代化的纹影光学系统对蛋白质条带进行可视化跟踪。由于在染色过程中不会出现电泳图的失真，因此该方法适用于定量的电泳法和蛋白质条带的检测。这种光学器件还适用于检测通常不被观察到的各种背景现象。
A modernized schlieren optics system was applied to follow protein bands visually during polyacrylamide gel electrophoresis. This approach is suitable for quantitative measurement of electrophoresis as well as for detection of protein bands because of the absence of distortion of the electrophoretic pattern during the staining process. The optics are also suitable for the detection of various background phenomena which usually escape observation.