MIGRATION PATHWAYS OF RECIRCULATING MURINE B-CELLS AND CD4+ AND CD8+ LYMPHOCYTES-T
MIGRATION PATHWAYS OF RECIRCULATING MURINE B-CELLS AND CD4+ AND CD8+ LYMPHOCYTES-T
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DOI：
10.1002/aja.1001870405
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发表时间：
1990-04-01
影响因子：
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通讯作者：
WEISS, L
中科院分区：
文献类型：
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作者：
PELLAS, TC;WEISS, L
绘制了小鼠胸导管淋巴细胞（TDL） B细胞和CD4+、CD8+T细胞亚群的迁移路径。不论淋巴细胞亚群如何，脾脏累积的TDL比任何其他器官都多。脾脏x线自显像显示早期TDL在边缘区和红髓聚集。许多TDl在1小时内通过脾静脉排出红髓。剩余的TDL不是直接从邻近边缘区进入白髓，而是通过远端动脉周围淋巴鞘（dPALS）进入白髓。在dPALS中，T细胞沿着中央动脉近端迁移到近端鞘（pPALS），并通过深淋巴管离开白髓。B细胞离开dPALS进入淋巴结节（NOD），然后也通过深淋巴管退出。T细胞比B细胞更容易到达淋巴结。淋巴细胞通过高内皮小静脉（HEV）进入淋巴结。弥漫性皮层中CD4+ TDL的绝对浓度高于CD8+细胞。然而，CD8+ TDL在弥漫性皮层中的移动速度比CD4+ TDL快。B细胞从HEV向NOD迁移。T型和B型TDL均通过皮质窦、髓窦和传出淋巴管排出。通过髓索的迁移途径被描述。所有的TDL子集都能很好地归巢到Peyer的补丁上。T - TDL从HEV转移到瘤旁区，而B细胞从HEV转移到NOD。所有TDL均经淋巴管排出。很少有TDL进入圆顶上皮下的区域。所有亚群都在圆顶上皮推定的M细胞的凹痕中观察到。
Migration pathways of B cell and CD4+ and CD8+T cell subsets of murine thoracic duct lymphocytes (TDL) were mapped. Per weight, the spleen accumulated more TDL than any other organ, regardless of lymphocyte subset. Spleen autoradiographs showed early accumulations of TDL in marginal zone and red pulp. Many TDl exited the red pulp within 1 hr via splenic veins. The remaining TDL entered the white pulp, not directly from the adjacent marginal zone but via distal periarterial lymphatic sheaths (dPALS). From dPALS, T cells migrated proximally along the central artery into proximal sheaths (pPALS) and exited the white pulp via deep lymphatic vessels. B cells left dPALS to enter lymphatic nodules (NOD), then also exited via deep lymphatics. T cells homed to lymph nodes more efficiently than B cells. Lymphocytes entered nodes via high-endothelial venules (HEV). CD4+ TDL reached higher absolute concentrations in diffuse cortex than did CD8+ cells. However, CD8+ TDL moved more quickly through diffuse cortex than did CD4+ TDL. B cells migrated from HEV into NOD. Both T and B TDL exited via cortical and medullary sinuses and efferent lymphatics. A migration pathway across medullary cords is described. All TDL subsets homed equally well to Peyer''s patches. T TDL migrated from HEV into paranodular zones while B cells moved from HEV into NOD. All TDL exited via lymphatics. Few TDL entered zones beneath dome epithelium. All subsets were observed within indentations in presumptive M cells of the dome epithelium.