Stable isotope signatures of underground seedlings reveal the organic matter gained by adult orchids from mycorrhizal fungi
Stable isotope signatures of underground seedlings reveal the organic matter gained by adult orchids from mycorrhizal fungi
复制标题
地下幼苗的稳定同位素特征揭示了成年兰花从菌根真菌中获得的有机物质
DOI：
10.1111/1365-2435.13042
复制
发表时间：
2018
影响因子：
5.2
通讯作者：
G. Gebauer
中科院分区：
文献类型：
--
作者：
Julienne M.‐I. Schweiger;M. Bidartondo;G. Gebauer
1. 兰花在其早期发育阶段依赖于菌根真菌提供的有机碳产生粉尘种子。因此，所有的叶绿素兰花在个体发育过程中都经历了从最初的分枝异养到自养或部分分枝异养的营养策略的巨大转变。然而，根据现有的方法，部分分枝异养兰花从菌根真菌中获得碳的程度尚不清楚。2. 在这里，我们提出了一种新的方法来量化叶绿素成熟兰花菌根与根核菌共生的真菌来源的有机质增益，利用它们的完全分枝异养（FMH）幼苗在线性双源混合模型中的稳定同位素特征。3. 对7种兰花进行了田间萌发试验，测定了成熟兰花、地下幼苗和自养参考植物的碳、氮、氢稳定同位素自然丰度和氮浓度。4. 原位埋藏19 ~ 30个月后，5种兰花的发芽率差异较大，2种兰花的发芽率不高。平均而言，地下幼苗相对于成熟兰花富集13℃和15 N，氮浓度较高。以幼苗的平均富集因子ε 13c和ε 2h作为FMH终点，成熟兰花从菌根中获得的有机质增益为0.20%。5. 带有根丝胞菌的叶绿素兰花由于其最初的菌丝体异养苗期而倾向于部分的真菌异养营养。我们发现地下幼苗的碳和氢同位素丰度可以用于改进的混合模型，以确定成熟兰花中真菌来源的有机物的重要比例。
1. Orchids produce dust seeds dependent on the provision of organic carbon by mycorrhizal fungi for their early development stages. Hence, all chlorophyllous orchids experience a dramatic switch in trophic strategies from initial mycoheterotrophy to either autotrophy or partial mycoheterotrophy during ontogeny. Yet, the degree to which partially mycoheterotrophic orchids gain carbon from their mycorrhizal fungi is unclear based on existing approaches. 2. Here, we propose a novel approach to quantify the fungal-derived organic matter gain of chlorophyllous mature orchids mycorrhizal with rhizoctonia fungi, using the stable isotope signatures of their fully mycoheterotrophic (FMH) seedlings in a lin ear two-source mixing model. 3. We conducted a field germination experiment with seven orchid species and measured carbon, nitrogen and hydrogen stable isotope natural abundances and nitrogen concentrations of mature orchids, underground seedlings, and autotrophic references. 4. After in situ burial for 19–30 months, germination rates varied considerably among five orchid species and failed for two. On average, underground seedlings were enriched in 13 C and 15 N relative to mature orchids and had higher nitrogen concentrations. Using the mean enrichment factors ε 13 C and ε 2 H of seedlings as FMH endpoint, the organic matter gain derived by mature orchids from mycorrhizas was c. 20%. 5. Chlorophyllous orchids mycorrhizal with rhizoctonias are predisposed to partially mycoheterotrophic nutrition due to their initially mycoheterotrophic seedling stage. We show that the carbon and hydrogen isotope abundances of underground seedlings can be used in an improved mixing-model to identify a significant proportion of fungal-derived organic matter in mature orchids.
登录
查看更多内容
改进部分真菌异养植物养分增益估计的方法学方法
影响因子：
1.3
作者：
Preiss K;Gebauer G.
通讯作者：
Gebauer G.
DOI：
10.1111/nph.12688
发表时间：
2014-04
期刊：
The New phytologist
影响因子：
--
作者：
Marcus Stöckel;T. Těšitelová;J. Jersáková;M. Bidartondo;G. Gebauer
通讯作者：
Marcus Stöckel;T. Těšitelová;J. Jersáková;M. Bidartondo;G. Gebauer
影响因子：
3
作者：
Liebel, Heiko T.;Bidartondo, Martin I.;Gebauer, Gerhard
通讯作者：
Gebauer, Gerhard
影响因子：
4.2
作者：
Hynson, Nicole A.;Schiebold, Julienne M. -I.;Gebauer, Gerhard
通讯作者：
Gebauer, Gerhard