Factors associated with the diversification of the microbial communities within different natural and artificial saline environments
Factors associated with the diversification of the microbial communities within different natural and artificial saline environments
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DOI：
10.1016/j.ecoleng.2015.06.029
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发表时间：
2015-10
影响因子：
3.8
通讯作者：
Shilong He;Qigui Niu;Y. Li;Yulun Nie;M. Hou
中科院分区：
文献类型：
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作者：
Shilong He;Qigui Niu;Y. Li;Yulun Nie;M. Hou
为探讨天然和工业盐环境中是否存在核心微生物群落，以及盐度对微生物群落的影响，采用构建16 SrRNA基因克隆文库的方法，对采自中国不同地区的2个天然样品和4个人工样品进行了分析。结果表明，天然样品的耐盐菌和嗜盐菌多样性显著高于人工样品。共检测到9个门，但只有拟杆菌和变形杆菌是常见的。典范对应分析表明，群落变异与Cl−、DOC、电导率和pH值密切相关。此外，Q-PCR结果显示，盐度影响微生物群落结构，细菌、古菌、真菌和酵母菌的数量逐渐减少。变异分配分析表明，DOC/Cl−对细菌种群变异的贡献最大，揭示了在含盐废水处理应用中用于功能恢复的核心控制因素。
To investigate whether there is a core microbial community of natural and industrial saline environments and to evaluate the effects of salinity on microbial communities, two natural samples and four artificial samples taken from different locations in China were analyzed by constructing 16S rRNA gene cloning libraries. The results showed that natural samples have a significantly higher halotolerant and halophilic bacterial diversity compared to artificial samples. In total, 9 phyla were detected among the samples, but onlyBacteroidetesandProteobacteriawere commonly shared. The canonical correspondence analysis demonstrated that the community variance correlated strongly with Cl−, DOC, conductivity, and pH. Moreover, the Q-PCR results revealed that salinity influenced the microbial community structure and progressively decreased the populations of bacteria, archaea, fungi and yeast. Variation partitioning analysis illustrated that DOC/Cl−had the greatest contribution to bacterial population variation, revealing a core control factor used for functional resilience in saline wastewater treatment applications.