Diversity of the oral microbiome between dentate and edentulous individuals
Diversity of the oral microbiome between dentate and edentulous individuals
复制标题
DOI：
10.1111/odi.13039
复制
发表时间：
2019-04-01
期刊：
影响因子：
3.8
通讯作者：
Schwartz, Joel L.
中科院分区：
文献类型：
--
作者：
Gazdeck, R. Kyle;Fruscione, Sarah R.;Schwartz, Joel L.
唾液微生物的测量被推广为检测口腔和全身性疾病的一种方法，但影响口腔微生物组的因素很多。唾液微生物组的脱落（上皮）和非脱落（牙齿）surfaces.Methods口腔生物膜的影响，以衡量唾液微生物分析的能力，以区分无牙和齿状口腔条件，我们寻找不同的唾液微生物组的受试者和没有牙齿。52名有牙和49名无牙受试者提供了刺激唾液样本。16S rRNA基因测序，QIIME为基础的数据处理，并进行统计分析，使用几种不同的分析方法来检测两组之间的唾液微生物组的差异。值得注意的是，所有31个最显着的差异类群的赤字，发生在缺牙组。正如人们所期望的那样，这些分类群中的许多都归因于牙菌斑和牙龈沟相关的bacteries.Conclusion总之，唾液中细菌的16S rRNA基因的测量可以用于可重复地测量与无牙症发生的口腔微生物组的差异，主要是缺乏牙齿和牙齿相关的结构。
Background Measurement of saliva microbes is promoted as a way to detect oral and systemic disease, yet there is a multitude of factors that affect the oral microbiome. The salivary microbiome is influenced by oral biofilm of shedding (epithelial) and non-shedding (tooth) surfaces.Methods To gauge the ability of salivary microbial analytics to distinguish between edentulous and dentate oral conditions, we looked for differences in the saliva microbiome of subjects with and without teeth. Fifty-two dentate and 49 edentulous subjects provided stimulated saliva samples. 16S rRNA gene sequencing, QIIME-based data processing, and statistical analysis were done using several different analytical approaches to detect differences in the salivary microbiome between the two groups.Results Bacteria diversity was lower in the edentulous group. Remarkably, all 31 of the most significant differences in taxa were deficits that occur in the edentulous group. As one might expect many of these taxa are attributed to dental plaque and gingival sulcus associated bacteria.Conclusion In sum, the measurement of 16S rRNA genes in the bacteria of the saliva can be used to reproducibly measure differences in the oral microbiome that occur with edentulism, mainly the lack of tooth and tooth-related structures.