Chemotaxins in cotton mill dust: possible etiologic agent(s) in byssinosis.
Chemotaxins in cotton mill dust: possible etiologic agent(s) in byssinosis.
复制标题
棉纺厂粉尘中的趋化因子：棉尘病的可能病原体。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
R. E. Neuman
中科院分区：
文献类型：
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作者：
S. Ainsworth;R. E. Neuman
通过应用简化的多形核白细胞趋化性体外测定，发现棉纺厂灰尘提取物中存在有效的趋化素。这些物质可能与肺表面细胞的募集以及吸入棉尘的纺织工人的棉尘病的病因有关。用碱性水溶液从棉尘中提取趋化素，并通过纸层析和Sephadex G-25 纯化。这些活性剂易溶于水，在100摄氏度的水中加热稳定，在水解条件下失活，阴离子，无荧光，分子量为200至2,000道尔顿。 Lacinilene 是棉尘中的一种荧光成分，据其他人报道具有趋化性，但由于这些特性而被排除在外，并且不会刺激从多种动物物种获得的白细胞的迁移。 Pluronic 多元醇 F68 是一种用作分散剂的聚丙二醇，被发现代表了一类新的趋化动力学物质，并且可能解释了早期关于 lacinilene 趋化活性的报道。
Through the application of a simplified in vitro assay for chemotaxis of polymorphonuclear leukocytes, potent chemotaxins have been found to exist in cotton mill dust extracts. These substances may be involved in the recruitment of cells to pulmonary surfaces and in the etiology of byssinosis in textile workers who inhale cotton dust. The chemotaxins were extracted from cotton dust with alkaline aqueous solution and purified by chromatography on paper and Sephadex G-25. The active agents were readily water-soluble, stable to heat at 100 degrees C in water, inactivated by hydrolytic conditions, anionic, nonfluorescent, and had molecular weights of 200 to 2,000 daltons. Lacinilene, a fluorescent component of cotton dust reported by others to be chemotactic, was excluded by these properties and did not stimulate migration of leukocytes obtained from a variety of animal species. Pluronic polyol F68, a polypropylene glycol used as a dispersing agent, was found to represent a new class of chemokinetic substances and may account for earlier reports of chemotactic activity of lacinilene.