Spectrum of mutations produced by specific types of restriction enzyme-induced double-strand breaks.
Spectrum of mutations produced by specific types of restriction enzyme-induced double-strand breaks.
复制标题
由特定类型的限制性内切酶诱导的双链断裂产生的突变谱。
DOI：
10.1093/mutage/7.6.439
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发表时间：
1992
期刊：
影响因子：
2.7
通讯作者：
Morgan,WF
中科院分区：
文献类型：
--
作者：
Winegar,RA;Lutze,LH;Rufer,JT;Morgan,WF
DNA双链断裂（DSB）的重新连接在导致DNA重排的各种过程中起着核心作用。我们已经分析了由产生具有特定类型末端的DSB的限制性内切酶诱导的DNA改变。将限制性内切酶电穿孔到稳定维持pHAZE的人淋巴母细胞系中，pHAZE是一种含有lacZ基因的基于EBV的载体。在允许时间进行DSB修复后，拯救pHAZE DNA并在大肠杆菌中筛选。pHAZE突变拷贝的作图和序列分析表明，限制性内切酶诱导除碱基置换（碱基缺失和插入、大规模缺失、倒位和插入）以外的所有类型的改变。光谱的改变是独特的每种酶，似乎是特定的末端修饰过程的结果。
Rejoining of DNA double-strand breaks (DSB) plays a central role in the various processes leading to DNA rearrangements. We have analyzed DNA alterations induced by restriction enzymes that produce DSB with specific types of ends. Restriction enzymes were electroporated into a human lymphoblastoid cell line that stably maintains pHAZE, an EBV-based vector containing thelacZgene. After allowing time for DSB repair, pHAZE DNA was rescued and screened inEscherichia coli. Mapping and sequence analysis of mutant copies of pHAZE indicated that restriction enzymes induced all classes of alterations except base substitutions (base deletions and insertions, large-scale deletions, inversions, and insertions). The spectra of alterations were distinctive for each enzyme and appear to be the consequence of specific end-modification processes.