Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping.
Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping.
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DOI：
10.1186/s12931-017-0555-4
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发表时间：
2017-04-20
影响因子：
5.8
通讯作者：
Inase N
中科院分区：
文献类型：
--
作者：
Shirai T;Furusawa H;Furukawa A;Ishige Y;Uchida K;Miyazaki Y;Eishi Y;Inase N
禽抗原是过敏性肺炎（HP）的常见病因。鸽血清和鸽滴提取物（PDE）吸入激发可诱发鸟类相关性过敏性肺炎（BRHP）患者的过敏反应，但这些材料中的抗原成分尚未完全阐明。用鸽血清、鸽肠匀浆和PDE与8例BRHP患者、2例夏季型HP患者、2例湿化肺患者和3例健康志愿者的血清样品进行免疫印迹。在鸽子血清和PDE中发现的蛋白质点中，通过质谱法鉴定了与BRHP患者血清反应的蛋白质点。使用重组蛋白进行免疫测定以确认所鉴定的蛋白的抗原性。还评估了用重组蛋白刺激的外周血单核细胞（PBMC）的细胞因子产生。免疫球蛋白类多肽-1（IGLL-1）从鸽血清和PDE的2-DE免疫印迹上的所有斑点中鉴定出来。BRHP患者表现出更高水平的血清IgG抗体对重组IGLL-1（rIGLL-1）相比，对照组，以及更强的PBMC增殖反应rIGLL-1。暴露于rIGLL-1后BRHP患者PBMC产生的细胞因子表明，该蛋白质可诱导Th 1倾向性免疫应答：TNF-α增加，IL-10产生不增加。鸽IGLL-1被鉴定为存在于鸽血清和PDE两者中的BRHP抗原。本文的在线版本（doi：10.1186/s12931-017-0555-4）包含补充材料，可供授权用户使用。
Avian antigen is a common cause of hypersensitivity pneumonitis (HP). Inhalation challenge with pigeon serum and pigeon dropping extract (PDE) elicits a hypersensitivity reaction in patients with bird-related hypersensitivity pneumonitis (BRHP), but the antigenic components in these materials have yet to be fully elucidated. Pigeon serum, pigeon intestine homogenates, and PDE were immunoblotted with serum samples from 8 patients with BRHP, 2 patients with summer-type HP, 2 patients with humidifier lung, and 3 healthy volunteers. Among the protein spots found in both pigeon serum and PDE, those that reacted with sera from BRHP patients were identified by mass spectrometry. Immunoassays using recombinant protein were performed to confirm the antigenicity of the identified protein. Cytokine production by peripheral blood mononuclear cells (PBMCs) stimulated with recombinant protein was also assessed. Immunoglobulin lambda-like polypeptide-1 (IGLL-1) was identified from all spots on 2-DE immunoblots of both pigeon serum and PDE. The BRHP patients exhibited higher levels of serum IgG antibody against the recombinant IGLL-1 (rIGLL-1) compared to the control subjects, as well as a stronger PBMCs proliferative response to rIGLL-1. Cytokine production by PBMCs from BRHP patients after rIGLL-1 exposure indicated that the protein could induce Th1 prone immune responses: an increase in TNF-α and an absence of elevated IL-10 production. Pigeon IGLL-1 was identified as the BRHP antigen present in both pigeon serum and PDE. The online version of this article (doi:10.1186/s12931-017-0555-4) contains supplementary material, which is available to authorized users.