ISOLATION AND CHARACTERIZATION OF ALGINATE-DERIVED OLIGOSACCHARIDES WITH ROOT GROWTH-PROMOTING ACTIVITIES
ISOLATION AND CHARACTERIZATION OF ALGINATE-DERIVED OLIGOSACCHARIDES WITH ROOT GROWTH-PROMOTING ACTIVITIES
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DOI：
10.1016/0008-6215(94)84085-7
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发表时间：
1994-05-20
影响因子：
3.1
通讯作者：
ADACHI, T
中科院分区：
文献类型：
--
作者：
NATSUME, M;KAMO, Y;ADACHI, T
使用来自Alteromonas macleodii的藻酸盐裂解酶裂解消化聚（甘露糖醛酸）、聚（古洛糖醛酸）和藻酸盐以制备不饱和寡糖的混合物。通过Q-Sepharose上的阴离子交换层析从海藻酸裂解酶裂解物中分离出四种寡糖，发现它们是促进根生长的裂解物的主要成分。通过NMR和西姆斯分析寡糖，并鉴定为在非还原末端具有O-（4-脱氧-L-乙硫基-己-4-烯吡喃糖醛酸）-1 ->的二糖和三糖。在大麦生物测定中，发现来自裂解物的三肽具有促进根生长的活性。
Lytic digestion of poly(mannuronate), poly(guluronate), and alginate with an alginate lyase from Alteromonas macleodii was used to prepare mixtures of unsaturated oligosaccharides. Four oligosaccharides isolated from the alginate lyase-lysate by anion-exchange chromatography on Q-Sepharose were found to be the major components of the root growth-promoting lysate. The oligosaccharides were analyzed by NMR and SIMS and identified as di- and tri-saccaharides having O-(4-deoxy-L-elythro-hex-4-enopyranosyluronic acid)-1 --> at the nonreducing terminus. The trisaccharides from the lysate were found to have root growth-promoting activity in a barley bioassay.