Cycling S-phase cells in animal and spontaneous tumours. I. Comparison of the BrdUrd and 3H-thymidine techniques and flow cytometry for the estimation of S-phase frequency.
Cycling S-phase cells in animal and spontaneous tumours. I. Comparison of the BrdUrd and 3H-thymidine techniques and flow cytometry for the estimation of S-phase frequency.
复制标题
动物和自发性肿瘤中 S 期细胞的循环。
DOI：
10.3109/02841868509134429
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
W. Göhde
中科院分区：
文献类型：
--
作者：
E. M. Langer;H. Röttgers;M. Schliermann;E. Meier;H. Miltenburger;J. Schumann;W. Göhde
Evaluation of the proliferative activities of cell populations has mainly been restricted to the use of autoradiography and flow cytometric measurements. The introduction of a new BrdUrd specific antibody makes it possible to determine exactly the DNA synthesizing cells. The BrdUrd technique is safe with respect to handling and the results are obtained within five hours. The suitability of the BrdUrd labelling procedure has been studied in different cell lines and compared with 3H-thymidine autoradiography and flow cytometry.
