Circulating CD4+CD25highFoxP3+T cells vary in different clinical forms of leprosy
Circulating CD4+CD25highFoxP3+T cells vary in different clinical forms of leprosy
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DOI：
10.1111/j.1365-4632.2010.04535.x
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发表时间：
2010-10-01
影响因子：
3.6
通讯作者：
Ali, Hala B.
中科院分区：
文献类型：
--
作者：
Attia, Enas A. S.;Abdallah, Marwa;Ali, Hala B.
据报道，cd4 + CD25highFoxP3+调节性T细胞（T-regs）在慢性感染中增加。我们的目的是研究它们在麻风中的频率，以探讨它们在麻风分枝杆菌感染中的作用。方法采用流式细胞术检测38例麻风患者和38例健康对照者外周血T-regs的频率和FoxP3的表达。患者分为；ⅰ组结核样（TT）、ⅱ组交界性病例[交界性结核样（BT）、交界性（BB）、交界性麻风样（BL）]、ⅲ组麻风样（LL）、ⅳ组麻风结节性红斑（ENL）。结果患者（特别是TT）的T-regs%和FoxP3的平均表达比对照组显著升高（分别为3.8 +/- 2.5%比2.5 +/- 0.8%和78.8 +/- 56.2%比55.8 +/- 15.7%）（P < 0.05）。4种疾病组比较，T-regs%差异有统计学意义（I组中位数为5.3%，II组中位数为3.4%，III组中位数为2.8%，IV组中位数为1.2%,P = 0.005）。T-regs foxp3在两组间差异无统计学意义[TT组中位数为71.5%，边缘组中位数为62.3%，LL组中位数为67.75%，ENL组中位数为85.75%；P = 0.149)。ENL中FoxP3的表达明显高于对照组（P = 0.011）。结论TT患者循环T-regs频率及抑制指标均升高。LL和ENL患者的T-regs表达频率显著降低，而FoxP3表达频率（在ENL中）显著升高，这与这些疾病类别的疾病进展和免疫超激活一致。因此，完整的T-regs活性可能对麻风病患者有益，而不是对免疫有害。
BackgroundCD4+ CD25highFoxP3+ regulatory T cells (T-regs) were reported to increase in chronic infections. We aimed at studying their frequency in leprosy to investigate their role during Mycobacterium leprae infection.MethodsUsing flow cytometry, the frequency and FoxP3 expression of circulating T-regs was assessed in 38 leprosy patients and 38 healthy controls. Patients were divided into; group I tuberculoid (TT), group II borderline cases [borderline tuberculoid (BT), borderline (BB), and borderline lepromatous (BL)], group III lepromatous (LL), and group IV erythema nodosum leprosum (ENL).ResultsMean T-regs% and FoxP3 expression were significantly elevated in patients (particularly TT) compared to controls (3.8 +/- 2.5% vs. 2.5 +/- 0.8% and 78.8 +/- 56.2% vs. 55.8 +/- 15.7%, respectively) (P < 0.05). Comparing the four disease groups, T-regs% was significantly different (median 5.3% in group I, 3.4% in group II, 2.8% in group III, and 1.2% in group IV; P = 0.005). FoxP3% on T-regs was not significantly different between them [median 71.5% in TT, 62.3% in borderline categories, 67.75% in LL, and 85.75% in ENL; P = 0.149). Notably FoxP3 expression was significantly higher in ENL than controls (P = 0.011).ConclusionThe frequency and suppressive marker of circulating T-regs are elevated in TT patients. Patients with LL and ENL express significantly lower frequency of T-regs and higher FoxP3 expression (in ENL), consistent with disease progression and immune hyper-activation in these disease categories. Thus, rather than being detrimental to immunity, intact T-regs activity may be beneficial to leprosy patients.