Periodic Melting of Oligonucleotides by Oscillating Salt Concentrations Triggered by Microscale Water Cycles Inside Heated Rock Pores
Periodic Melting of Oligonucleotides by Oscillating Salt Concentrations Triggered by Microscale Water Cycles Inside Heated Rock Pores
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DOI：
10.1002/anie.201907909
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发表时间：
2019-09-09
影响因子：
16.6
通讯作者：
Braun, Dieter
中科院分区：
文献类型：
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作者：
Ianeselli, Alan;Mast, Christof B.;Braun, Dieter
为了理解生命的出现，更好地理解原始非平衡条件的物理化学是必不可少的。RNA的催化功能需要大量的盐浓度。将寡核苷酸分离成单链是一个困难的问题，因为RNA的水解在高温下成为一个限制因素。盐浓度调节DNA或RNA的熔化，它的周期性调节将使熔化和退火循环在较低温度下进行。在我们的实验中，适度的温差产生了一个小型化的水循环，导致盐浓度的波动，导致寡核苷酸在低于熔化温度20摄氏度的温度下熔化。这将使连接链复制所必需的双链寡核苷酸重新洗牌。这些发现表明，在早期进化中，有一种自主的方法来克服非酶复制的链分离问题。
To understand the emergence of life, a better understanding of the physical chemistry of primordial non-equilibrium conditions is essential. Significant salt concentrations are required for the catalytic function of RNA. The separation of oligonucleotides into single strands is a difficult problem as the hydrolysis of RNA becomes a limiting factor at high temperatures. Salt concentrations modulate the melting of DNA or RNA, and its periodic modulation would enable melting and annealing cycles at low temperatures. In our experiments, a moderate temperature difference created a miniaturized water cycle, resulting in fluctuations in salt concentration, leading to melting of oligonucleotides at temperatures 20 degrees C below the melting temperature. This would enable the reshuffling of duplex oligonucleotides, necessary for ligation chain replication. The findings suggest an autonomous route to overcome the strand-separation problem of non-enzymatic replication in early evolution.