Model surfaces engineered with nanoscale roughness and RGD tripeptides promote osteoblast activity
Model surfaces engineered with nanoscale roughness and RGD tripeptides promote osteoblast activity
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DOI：
10.1002/jbm.a.20051
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发表时间：
2004-03-15
影响因子：
4.9
通讯作者：
Composto, RJ
中科院分区：
文献类型：
--
作者：
El-Ghannam, AR;Ducheyne, P;Composto, RJ
细胞粘附到生物材料是组织与植入物表面整合的先决条件。在这里，我们表明我们可以生成一个模型二氧化硅表面，其中包含最小长度的精氨酸-甘氨酸-天冬氨酸（RGD）肽，该肽保持其生物活性。在本研究的第一部分中，研究了MC 3 T3-E1成骨细胞样细胞在氧化硅、胺封端的底物[即，3-氨基丙基三乙氧基硅烷（APTS）]、接枝的RGD和物理吸附的RGD对照。APTS层表现出纳米级的粗糙度，并提出了胺官能团，用于接枝没有任何侧翼基团或间隔基的最小的RGD三肽。接触角的测量表明，APTS表面的疏水性显着低于表面接枝RGD（RGD-APTS）。原子力显微镜显示覆盖有RGD-APTS的表面比单独覆盖有APTS的表面更光滑（Ra = 0.71 nm）（Ra = 1.59 nm）。主要集中在细胞形态，实验表明，RGD-APTS混合提供了一个最佳的表面细胞粘附，传播，和细胞骨架组织。还观察到与成功的细胞信号传导事件一致的离散粘着斑。在第二组实验中，使用APTS的光滑单层（Ra = 0.1 nm）制备甘氨酸-甘氨酸-天冬氨酸-丝氨酸（RGDS）-APTS和甘氨酸-甘氨酸-谷氨酸-丝氨酸（RGES）-APTS（对照）底物。主要集中在细胞功能，整合素和基因表达都增加了率骨肉瘤细胞表面含有移植RGDS。两组研究都表明，移植的RGD（S）分子增强成骨细胞样细胞的粘附和功能。(C)2004 Wiley Periodicals，Inc.
Cell adhesion to biomaterials is a prerequisite for tissue integration with the implant surface. Herein, we show that we can generate a model silica surface that contains a minimal-length arginine-glycine-aspartic acid (RGD) peptide that maintains its biological activity. In the first part of this study, attachment of MC3T3-E1 osteoblast-like cells was investigated on silicon oxide, amine terminated substrates [i.e., 3-aminopropyl triethoxysilane (APTS)], grafted RGD, and physisorbed RGD control. The APTS layer exhibited nanoscale roughness and presented amine functional groups for grafting a minimal RGD tripeptide devoid of any flanking groups or spacers. Contact angle measurements indicated that the hydrophobicity of the APTS surface was significantly lower than that of the surface with grafted RGD (RGD-APTS). Atomic force microscopy showed that surfaces covered with RGD-APTS were smoother (Ra = 0.71 nm) than those covered with APTS alone (Ra = 1.59 nm). Focusing mainly on cell morphology, experiments showed that the RGD-APTS hybrid provided an optimum surface for cell adhesion, spreading, and cytoskeletal organization. Discrete focal adhesion plaques were also observed consistent with successful cell signaling events. In a second set of experiments, smooth, monolayers of APTS (Ra = 0.1 nm) were used to prepare arginine-glycine-aspartic acid-serine (RGDS)-APTS and arginine-glycine-glutamic acid-serine (RGES)-APTS (control) substrates. Focusing mainly on cell function, integrin and gene expression were all enhanced for rate osteosarcoma cells on surfaces containing grafted RGDS. Both sets of studies demonstrated that grafted molecules of RGD(S) enhance both osteoblast-like cell adhesion and function. (C) 2004 Wiley Periodicals, Inc.