Arginine grafting to endow cell permeability
Arginine grafting to endow cell permeability
复制标题
DOI：
10.1021/cb600429k
复制
发表时间：
2007-03-01
影响因子：
4
通讯作者：
Raines, Ronald T.
中科院分区：
文献类型：
--
作者：
Fuchs, Stephen M.;Raines, Ronald T.
我们报告了一种赋予蛋白质渗透哺乳动物细胞的能力而不附加外源结构域的方法。我们的方法是通过嫁接精氨酸残基在目标蛋白的表面安装阳离子补片。使用 GFP 这样做不会损害构象稳定性，但可以实现依赖于细胞表面糖胺聚糖的有效细胞摄取。我们预计这种可渗透细胞的 GFP 变体无需转染，将可用于细胞生物学的众多应用，并且精氨酸移植方法将得到广泛应用。
We report on a means to endow proteins with the ability to permeate mammalian cells without appending an exogenous domain. Our approach is to install a cationic patch on the surface of a target protein by the grafting of arginine residues. Doing so with GFP did not compromise conformational stability but enabled efficient cellular uptake that was dependent on cell-surface glycosaminoglycans. We anticipate that this cell-permeable variant of GFP, which obviates the need for transfection, will be useful for numerous applications in cell biology and that the method of arginine grafting will be broadly applicable.