Rat preprocarboxypeptidase A: cDNA sequence and preliminary characterization of the gene.
Rat preprocarboxypeptidase A: cDNA sequence and preliminary characterization of the gene.
复制标题
大鼠前羧肽酶原 A：cDNA 序列和基因的初步表征。
DOI：
10.1073/pnas.79.1.31
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
Rutter,WJ
中科院分区：
文献类型：
--
作者：
Quinto,C;Quiroga,M;Swain,WF;NikovitsJr,WC;Standring,DN;Pictet,RL;Valenzuela,P;Rutter,WJ
大鼠羧肽酶 A cDNA 克隆已从胰腺 mRNA 制备的 cDNA 文库中分离出来。已推导出几乎完整的mRNA序列，其预测与牛羧肽酶A具有78％氨基酸序列同源性的多肽。从核苷酸序列推断羧肽酶A前体的激活肽和信号肽的氨基酸序列。该 cDNA 用作探针，以鉴定大鼠基因组 DNA 噬菌体 lambda 文库中含有羧肽酶 A 序列的 DNA 片段。异源双链体显示 DNA 编码序列占据 5.5 KB，并被 9 个插入序列打断。基因 5' 末端和相邻侧翼区域的核苷酸序列提供了有关转录起始位点和推定控制区域的信息。基因中插入序列的定位与蛋白质的功能/结构域之间没有明显的关系。
Rat carboxypeptidase A cDNA clones have been isolated from a cDNA library prepared from pancreatic mRNA. An almost complete mRNA sequence has been deduced that predicts a polypeptide having 78% amino acid sequence homology with bovine carboxypeptidase A. The amino acid sequence of the activation and signal peptides of the carboxypeptidase A precursor were inferred from the nucleotide sequence. The cDNA was used as a probe to identify DNA fragments containing carboxypeptidase A sequences in a bacteriophage lambda library of rat genomic DNA. Heteroduplexes revealed that the DNA coding sequence occupies 5.5 kilobases and is interrupted by nine intervening sequences. The nucleotide sequence of the 5' end of the gene and the adjacent flanking region provides information on the site of initiation of transcription and the putative control regions. There is no evident relationship between the localization of intervening sequences in the gene and functional/structural domains of the protein.