Unique therapeutic effects of the Japanese-Chinese herbal medicine, Sairei-to, on Th1/Th2 cytokines balance of the autoimmunity of MRL/lpr mice
Unique therapeutic effects of the Japanese-Chinese herbal medicine, Sairei-to, on Th1/Th2 cytokines balance of the autoimmunity of MRL/lpr mice
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DOI：
10.1016/s0923-1811(01)00161-x
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发表时间：
2002-04-01
影响因子：
4.6
通讯作者：
Furukawa, F
中科院分区：
文献类型：
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作者：
Ito, T;Seo, N;Furukawa, F
斋玲岛日本-中国草药之一已被用于治疗各种疾病，特别是胶原病和肾病综合征中的水肿。然而.治疗作用的机制仍然不确定。因此。我们观察了自身免疫易感型MRL lpr的皮肤、肾脏、脾细胞和脾脏的免疫学变化。MRL/n和C57 BL 6 J小鼠。在MRL lpr小鼠中，用Sairei-to治疗，蛋白尿的改善。观察到肾脏中苏木素体数量减少和血尿素氮血清水平降低。这些结果表明，Sairei-to可以改善或抑制狼疮性肾炎的进展。CD 19比例和血清IgG 1水平。是狼疮性皮肤病和狼疮性肾炎的发病机制之一。在Sairei处理的MRL lpr小鼠中显著降低。因此，怀疑B细胞功能被赛灵汤抑制。此外，MRL/lpr小鼠脾细胞CD 4 8比值和淋巴细胞增殖程度也降低。很有趣。ELISPOT检测显示，Sairei诱导的MRL lpr小鼠脾细胞产生IL-4的能力显著增强，IFN-γ mRNA表达降低。调节Th平衡。Th 1优势失衡可能在MRL lpr小鼠中起重要作用，Sairei处理的MRL lpr小鼠中Th 1轴被抑制，Th 2轴成为优势。从另一方面来说。Th 2细胞型免疫球蛋白（IgG 1）被抑制，这些结果表明，Sairei-to可能会损害Th 1 Th 2平衡和高丙种球蛋白血症，从而产生治疗效果，（C）2002 Elsevier Science爱尔兰Ltd.保留所有权利。
Sairei-to. one of the Japanese-Chinese herbal medicines has been used for the treatment of various diseases, especially collagen disease and edema in nephrotic syndrome. However. the mechanism of the therapeutic effects remains uncertain. Therefore. we investigated the immunological changes of skin, kidney, spleen cells and scrum in autoimmune-prone MRL lpr. MRL/n and C57BL 6J mice treated with Sairei-to. In MRL lpr mice treated with Sairei-to, the improvement of proteinuria. reduction in the number of hematoxylin bodies in kidney, and reduced serum levels of blood urea nitrogen were observed. These results indicate that Sairei-to can improve or inhibit file progression of lupus nephritis. The proportion of CD19 and the Serum levels of IgG1. which is one of the pathogenesis of lupus dermatoses and lupus nephritis. were significantly reduced in Sairei-to-treated MRL lpr mice. Therefore, it is suspected that the B cell function was suppressed by Sairei-to. In addition, CD4 8 ratio in spleen cells and the degree of lymphoproliferation in MRL/lpr mice also decreased. Interestingly. IL-4 producing spleen cells were increased significantly by ELISPOT assay, and IFN-gamma mRNA expressions were reduced in Sairei-to-reated MRL lpr mice. Regal-ding the Th balance. an imbalance towards Th1 predominance may, plan a significant role in MRL lpr mice, and the Th1 axis was suppressed and the Th2 axis became predominant in Sairei-to-treated MRL lpr mice. On the other hand. Th2 cell type immunoglobulins (IgG1) were Suppressed, These results suggested that Sairei-to is potential for impairing shifted Th1 Th2 balance and hypergammablobulinemia resulting in therapeutic effects, (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.