Delftia lacustris sp nov., a peptidoglycan-degrading bacterium from fresh water, and emended description of Delftia tsuruhatensis as a peptidoglycan-degrading bacterium
Delftia lacustris sp nov., a peptidoglycan-degrading bacterium from fresh water, and emended description of Delftia tsuruhatensis as a peptidoglycan-degrading bacterium
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DOI：
10.1099/ijs.0.008375-0
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发表时间：
2009-09-01
影响因子：
2.8
通讯作者：
Hansen, Michael
中科院分区：
文献类型：
--
作者：
Jorgensen, Niels O. G.;Brandt, Kristian K.;Hansen, Michael
细胞外肽聚糖广泛存在于自然环境中，但其生物降解机制却知之甚少。我们在这里描述了一种新的肽聚糖降解细菌，命名为菌株332（T），分离自丹麦的中营养湖水。该菌株为革兰氏阴性染色的活动杆状菌。它具有几丁质酶和溶菌酶活性，这与肽聚糖降解有关，并且能够利用几种单糖和二糖、氨基酸和有机酸。基于16 S rRNA基因序列的系统发育分析表明，菌株332（T）属于Delftia属。该菌株的脂肪酸包括C-8：0和C-10：0，这是Delftia属的特征。DNA G + C含量为65.3mol%。菌株332（T）与Delftia tsuruhatensis DSM 17581（T）的DNA杂交率为66.2%。基于生理生化特征的差异，该菌株被认为是代表一个新种，并建议将其命名为Delftia lacustris sp. nov.。模式菌株为332（T）（=DSM 21246（T）=LMG 24775（T））。本文还提出了一种对鹤鹤黛香Delftia tsuruhatensis的描述。
Extracellular peptidoglycan is commonly found in natural environments, yet little is known about its biodegradation in nature. We here describe a novel peptidoglycan-degrading bacterium, designated strain 332(T), isolated from mesotrophic lake water in Denmark. The strain was a Gram-negative-staining, motile rod. It had chitinase and lysozyme activities, which are relevant to peptidoglycan degradation, and was capable of utilizing several mono- and disaccharides, amino acids and organic acids. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain 332(T) belonged to the genus Delftia. Fatty acids of the strain included C-8:0 and C-10:0, which are characteristic of the genus Delftia. The DNA G + C content of the strain was 65.3 mol%. A DNA-DNA hybridization value of 66.2% was found between strain 332(T) and Delftia tsuruhatensis DSM 17581(T). Based on differences in physiological and biochemical characteristics, the strain is considered to represent a novel species, for which the name Delftia lacustris sp. nov. is proposed. The type strain is 332(T) (=DSM 21246(T) =LMG 24775(T)). An emended description of Delftia tsuruhatensis is also presented.