Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells
Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells
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DOI：
10.1359/jbmr.1999.14.9.1486
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发表时间：
1999-09-01
影响因子：
6.2
通讯作者：
Kurihara, H
中科院分区：
文献类型：
--
作者：
Sakata, M;Shiba, H;Kurihara, H
骨保护素(OPG)/破骨细胞生成抑制因子(OCIF)抑制破骨细胞的分化、活性和存活，因此OPG/OCIF可能调节牙科硬组织(牙槽骨、牙骨质和牙本质)的吸收。为了研究这一问题，使用OPG/OCIF的特异引物进行逆转录聚合酶链式反应，从培养的人牙周角质形成细胞(HGKs)、人牙周成纤维细胞(HGFs)、人牙周韧带细胞(HPDL)和人牙髓细胞(HPC)中提取总RNA。在HGFs、HPDL和HPC中发现了PCR产物，而在HGKs中没有发现，并且这些产物的DNA序列与已报道的OPG基因序列100%一致。Northern印迹分析也表明，t1在HGFs、HPDL和HPC，而不是HGKs表达OPG/OCIF转录本，其大小与2.5kb相似。白介素1β(IL-1β)和肿瘤坏死因子-α(TNF-α)以剂量和时间依赖的方式增加HPDL中OPG/OCIF的mRNA水平。作用12h后，3 ng/mlIL-1β和3 ng/mlTNF-α分别使OPG/OCIF mRNA表达增加190%和110%，作用最强。IL-1β和TNF-α对HPC也有刺激作用。IL-6和转化生长因子-β对HPDL中OPG/OCIF基因的表达无明显影响。提示牙间充质细胞合成的OPG/OCIF通过细胞因子对牙体硬组织的吸收起局部调控作用。
Osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) inhibits osteoclast differentiation, activity, and survival; therefore OPG/OCIF may regulate the resorption of dental hard tissues, such as alveolar bone, cementum, and dentin, To investigate this issue, reverse transcriptase-polymerase chain reaction using specific primers for OPG/OCIF was performed,vith total RNAs isolated from human gingival keratinocytes (HGKs), human gingival fibroblasts (HGFs), human periodontal ligament cells (HPDLs), and human pulp cells (HPCs) in culture. PCR products were found in HGFs, HPDLs, and HPCs, but not in HGKs, and the DNA sequence of these products was 100% identical to the reported sequence of the OPG gene. Northern blot analyses also showed tl;at HGFs, HPDLs, and HPCs, but not HGKs, expressed OPG/OCIF transcripts of similar to 2.5 kb. Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) increased OPG/OCIF mRNA levels in a dose-and time-dependent manner in HPDL. After 12 h of treatment, IL-1 beta at 3 ng/ml and TNF-alpha at 3 ng/ml increased OPG/OCIF mRNA expression by 190% and 110%, respectively,,vith a maximal effect. The stimulatory effects of IL-1 beta and TNF-alpha were also seen in HPC. However, IL-6 and transforming growth factor-beta had little effect on OPG/OCIF mRNA levels in HPDL. These findings suggest that OPG/OCIF synthesized by dental mesenchymal cells locally regulates the resorption of dental hard tissues through cytokines.