Discovery, evaluation and distribution of haplotypes of the wheat Ppd-D1 gene
Discovery, evaluation and distribution of haplotypes of the wheat Ppd-D1 gene
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DOI：
10.1111/j.1469-8137.2009.03099.x
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发表时间：
2010-02-01
期刊：
影响因子：
9.4
通讯作者：
Jia, Jizeng
中科院分区：
文献类型：
--
作者：
Guo, Zhiai;Song, Yanxia;Jia, Jizeng
Ppd-D1基因是影响小麦光周期反应最强的基因之一。本研究利用5个分子标记对来自不同地理区域的492份小麦品种和55份粗山羊草（Aegilops tauschii）的Ppd-D1单倍型进行了鉴定，共鉴定出6种Ppd-D1单倍型，其中Ppd-D1 a和Ppd-D1 b分别为10种和10种，Ppd-D1 a和Dpd-D1 b分别为10种和10种，Ppd-D1 a和Dpd-D1 b分别为10种和10种，Ppd-D1 b分别为10种和10种，Ppd-D1 a和Dpd-D1 b分别为10种和10种，Ppd-D1 a和Dpd-D1 b分别为10种和10种，Ppd-D1 a和Dpd-D1 b分别为10种和10种，Ppd-D1 a和Dpd-D1 b分别为10种和10种，Ppd-D1 b分别为1被称为I-VI。II、V和VI型被认为更古老，而I、III和IV型被认为是从II型衍生而来。Ppd-D1单倍型的转录本丰度呈连续变化，单倍型I最高，单倍型III最低，并与抽穗期的品种差异呈负相关。这些单倍型也显著影响其他农艺性状。Ppd-D1单倍型的分布频率与小麦栽培区的纬度和海拔高度均呈偏相关，Ppd-D1单倍型的进化、表达和分布具有明显的一致性。过去被认为是一对等位基因的东西，现在可以被认为是导致连续变异的一系列等位基因。
Ppd-D1 is one of the most potent genes affecting the photoperiod response of wheat (Triticum aestivum). Only two alleles, insensitive Ppd-D1a and sensitive Ppd-D1b, were known previously, and these did not adequately explain the broad adaptation of wheat to photoperiod variation.In this study, five diagnostic molecular markers were employed to identify Ppd-D1 haplotypes in 492 wheat varieties from diverse geographic locations and 55 accessions of Aegilops tauschii, the D genome donor species of wheat.Six Ppd-D1 haplotypes, designated I-VI, were identified. Types II, V and VI were considered to be more ancient and types I, III and IV were considered to be derived from type II. The transcript abundances of the Ppd-D1 haplotypes showed continuous variation, being highest for haplotype I, lowest for haplotype III, and correlating negatively with varietal differences in heading time. These haplotypes also significantly affected other agronomic traits. The distribution frequency of Ppd-D1 haplotypes showed partial correlations with both latitudes and altitudes of wheat cultivation regions.The evolution, expression and distribution of Ppd-D1 haplotypes were consistent evidentially with each other. What was regarded as a pair of alleles in the past can now be considered a series of alleles leading to continuous variation.